{"id":557,"date":"2024-04-14T22:07:00","date_gmt":"2024-04-14T14:07:00","guid":{"rendered":"https:\/\/blog.ineuro.net\/?p=557"},"modified":"2024-05-02T22:08:10","modified_gmt":"2024-05-02T14:08:10","slug":"%e6%95%b4%e5%90%88%e7%a9%ba%e9%97%b4%e8%bd%ac%e5%bd%95%e7%bb%84%e5%92%8c%e4%bb%a3%e8%b0%a2%e7%a0%94%e7%a9%b6%e6%8f%ad%e7%a4%ba%e4%ba%ba%e8%84%91%e6%8d%9f%e4%bc%a4%e5%90%8e%e7%9a%84%e4%bb%a3%e8%b0%a2","status":"publish","type":"post","link":"https:\/\/blog.ineuro.net\/index.php\/2024\/04\/14\/557\/","title":{"rendered":"\u6574\u5408\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u548c\u4ee3\u8c22\u7814\u7a76\u63ed\u793a\u4eba\u8111\u635f\u4f24\u540e\u7684\u4ee3\u8c22\u5f02\u8d28\u6027"},"content":{"rendered":"\n

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\"\u56fe\u7247[1]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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\"\u56fe\u7247[2]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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\"\u56fe\u7247[3]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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\"\u56fe\u7247[4]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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\u7814\u7a76\u4eba\u5458\u5148\u524d\u62a5\u9053\u8fc7\uff0c\u7ea2\u85fb\u6c28\u9178\u53ef\u4ee5\u8bf1\u5bfc\u556e\u9f7f\u52a8\u7269\u6d77\u9a6c\u795e\u7ecf\u5143\u4e27\u5931\u548c\u589e\u52a0mINS\u6c34\u5e73\uff0c\u8fd9\u662f\u766b\u75eb\u6301\u7eed\u72b6\u6001(post-status epilepticus, postSE)\u7684\u6a21\u578b\u3002\u518d\u6b21\uff0c\u7814\u7a76\u4eba\u5458\u53d1\u73b0\uff0c\u4e0e\u78f7\u9178\u76d0\u7f13\u51b2\u6db2(phosphate-buffered saline, PBS)\u6cbb\u7597\u7684\u5927\u9f20\u76f8\u6bd4\uff0c\u7ea2\u85fb\u6c28\u9178(KA)\u6cbb\u7597\u7684\u5927\u9f20NAA\/Cr\u964d\u4f4e\uff0cmINS\/Cr\u5347\u9ad8(\u56fe6A-6D)\u3002\u6b64\u5916\uff0c\u514d\u75ab\u7ec4\u5316(IHC)\u56fe\u50cf\u8bc1\u5b9e\u4e86\u5927\u9f20\u635f\u4f24\u8111\u7ec4\u7ec7\u4e2dS1PR5\u548cSEPTIN4\u7684\u86cb\u767d\u8868\u8fbe\u589e\u52a0(\u56fe6E)\u3002\u540c\u6837\uff0c\u5927\u9f20\u635f\u4f24\u8111\u7ec4\u7ec7\u4e2dSLC5A3\u548cSLC5A11\u7684\u8868\u8fbe\u4e5f\u901a\u8fc7IHC\u9a8c\u8bc1(\u56fe6E)\u3002<\/p>\n\n\n\n

\"\u56fe\u7247[5]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

\u901a\u8def\u5206\u6790\u8bc1\u5b9e\uff0c\u4e2d\u5ea6\u548c\u91cd\u5ea6TBI\u7ec4\u4e4b\u95f4\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u4e0e\u7ec6\u80de\u6740\u4f24\u548c\u4ee3\u8c22\u76f8\u5173(\u56fe6F\u548c6G)\uff0c\u800cGO\u5bcc\u96c6\u8868\u660e\u7ec6\u80de\u6740\u4f24\u548c\u6297\u6c27\u5316\u6d3b\u6027\u76f8\u5173(\u56fe6F)\u3002\u6709\u8da3\u7684\u662f\uff0cKEGG\u901a\u8def\u5b9a\u4e49\u4e86\u4e00\u7cfb\u5217\u4ee3\u8c22\uff0c\u5176\u7279\u5f81\u662f\u5916\u6e90\u751f\u7269\u964d\u89e3\u3001\u841c\u7c7b\u3001\u7ef4\u751f\u7d20\u3001\u8102\u8d28\u4ee3\u8c22\u3001\u805a\u7cd6\u751f\u7269\u5408\u6210\u3001\u80fd\u91cf\u4ee3\u8c22\u3001\u78b3\u6c34\u5316\u5408\u7269\u548c\u6c28\u57fa\u9178\u4ee3\u8c22(\u56fe6G)\u3002\u7531\u4e8e\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0(DEGs)\u4e0e\u7ec6\u80de\u6b7b\u4ea1\u3001\u6297\u6c27\u5316\u6d3b\u6027\u548c\u8102\u8d28\u4ee3\u8c22\u76f8\u5173\uff0c\u56e0\u6b64\u6709\u7406\u7531\u8ba4\u4e3a\u7a7a\u95f4\u57fa\u56e0\u7c07\u53ef\u4ee5\u4f5c\u4e3a\u53d7\u635f\u5927\u8111\u8fdb\u4e00\u6b65\u4ee3\u8c22\u53d8\u5316\u7684\u89e3\u7801\u5668\u3002<\/p>\n\n\n\n

\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u4e0e\u4ee3\u8c22\u7269\u4e4b\u95f4\u7684\u8054\u7cfb<\/h2>\n\n\n\n

\u9488\u5bf9\u4ee3\u8c22\u7269\u7684\u5177\u4f53\u5b9a\u91cf\u6307\u6807\uff0c\u7814\u7a76\u4eba\u5458\u7814\u7a76\u4e86SLC5A3\u3001SLC5A11\u4e0eMI\u6c34\u5e73\u7684\u7ebf\u6027\u5173\u7cfb\u3002\u9996\u5148\uff0c\u7814\u7a76\u4eba\u5458\u901a\u8fc7feature plot\u548cviolin plot\u5c55\u793a\u4e86SLC5A3\u548cSLC5A11\u7684\u7ec6\u80de\u5b9a\u4f4d\u3002
SLC5A3\u4e3b\u8981\u8868\u8fbe\u4e8e\u5185\u76ae\u7ec6\u80de\uff0c\u800cSLC5A11\u8868\u8fbe\u4e8e\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u3001\u5185\u76ae\u7ec6\u80de\u548c\u514d\u75ab\u7ec6\u80de(\u56fe7A-7D)\u3002\u540c\u65f6\uff0c\u4eceGeneCard\u7f51\u7ad9\u5f97\u77e5SLC5A3\u548cSLC5A11\u90fd\u662f\u808c\u9187\u548c\u8461\u8404\u7cd6\u7684\u8f6c\u8fd0\u4f53\uff0cSLC5A11\u4e0e\u7ec6\u80de\u51cb\u4ea1\u76f8\u5173\u3002
\u7814\u7a76\u4eba\u5458\u8fdb\u4e00\u6b65\u83b7\u5f97\u4e86\u8fd9\u4e24\u4e2a\u57fa\u56e0\u7684spot\u503c\uff0c\u5e76\u6bd4\u8f83\u4e86\u4e2d\u5ea6\u548c\u91cd\u5ea6TBI\u7ec4\u4e4b\u95f4\u7684\u5404\u8868\u8fbe\u91cf(\u56fe7E\u548c7F)\u3002\u7814\u7a76\u4eba\u5458\u53d1\u73b0\uff0c\u4e0e\u4e2d\u5ea6TBI\u7ec4\u76f8\u6bd4\uff0c\u91cd\u5ea6TBI\u7ec4\u7684SLC5A3\u76f8\u5bf9\u8868\u8fbe\u91cf\u8f83\u4f4e\uff0c\u800cSLC5A11\u7684\u8868\u8fbe\u91cf\u5219\u8f83\u9ad8(\u56fe7G\u548c7H)\u3002\u7814\u7a76\u4eba\u5458\u8fd8\u7814\u7a76\u4e86SLC5A3\u3001SLC5A11\u4e0e\u4e0d\u540c\u4ee3\u8c22\u7269\u4e4b\u95f4\u7684\u7ebf\u6027\u5173\u7cfb\u3002\u7814\u7a76\u4eba\u5458\u4ec5\u53d1\u73b0SLC5A11\u4e0eMI\u6c34\u5e73\u548cPC(44:7)\u6c34\u5e73\u5448\u6b63\u76f8\u5173\uff0c\u8fd9\u53ef\u80fd\u4e5f\u662f\u7531\u4e8e\u6837\u672c\u91cf\u5c0f\u6240\u81f4(\u56fe7I-7P)\u3002<\/p>\n\n\n\n

\"\u56fe\u7247[6]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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