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\u539f\u6587\uff1aBolte, Shapiro et al. eLife 2023;12:e81154. DOI: https:\/\/doi.org\/10.7554\/eLife.81154<\/a><\/p>\n\n\n\n

Sequencing data have been deposited in GEO under accession code GSE206941. All code used for analysis is available at https:\/\/github.com\/danielshapiro1\/MeningealTransciptome<\/a> (copy archived at swh:1:rev:d75b20d74f147524296630b9ec7a1c9c5a9f124f<\/a>). All data generated or analyzed during this study are included in the manuscript and supporting file; Source Data files have been provided for all figures.<\/p>\n\n\n\n

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\u4e8c\u3001\u7814\u7a76\u7ed3\u679c<\/h1>\n\n\n\n

2.1 \u8f7b\u5ea6\u9885\u8111\u635f\u4f24\u5f15\u53d1\u8111\u819c\u7ec6\u80de\u7ec4\u6210\u7684\u53d8\u5316\u3002<\/h2>\n\n\n\n

\u4e3a\u6df1\u5165\u4e86\u89e3\u9885\u8111\u635f\u4f24\u5982\u4f55\u5f71\u54cd\u8111\u819c\u751f\u7269\u5b66\uff0c\u6211\u4eec\u5bf9\u5c0f\u9f20\u8fdb\u884c\u4e86\u8f7b\u5ea6\u95ed\u9885\u635f\u4f24\uff0c\u5e76\u5728\u4f24\u540e\u4e00\u5468\u8fdb\u884c\u4e86\u8111\u819c\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff08\u56fe1a\uff09\u3002\u5728\u8fd9\u4e2a\u8f7b\u5ea6\u9885\u8111\u635f\u4f24\u6a21\u578b\u4e2d\uff0c\u5c0f\u9f20\u4f7f\u7528\u7acb\u4f53\u5b9a\u5411\u7535\u78c1\u649e\u51fb\u5668\u53d7\u5230\u4e00\u6b21\u6027\u51fb\u6253\u53f3\u4fa7\u989e\u53f6\u548c\u989d\u53f6\uff08\u56fe1\u2014\u9644\u56fe1a\uff1bBolte\u7b49\uff0c2020\u5e74\uff09\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u6211\u4eec\u5148\u524d\u5df2\u7ecf\u8bc1\u660e\u5728\u8fd9\u4e2a\u6a21\u578b\u4e2d\u7684\u5934\u90e8\u635f\u4f24\u4e0d\u4f1a\u5bfc\u81f4\u5e73\u8861\u3001\u8fd0\u52a8\u534f\u8c03\u3001\u53cd\u5c04\u548c\u8b66\u89c9\u6027\u7684\u660e\u663e\u6539\u53d8\uff08Bolte\u7b49\uff0c2020\u5e74\uff09\u3002\u4e0e\u8fd9\u4e2a\u8f7b\u5ea6\u9885\u8111\u635f\u4f24\u6a21\u578b\u7684\u6027\u8d28\u4e00\u81f4\uff0c\u6211\u4eec\u5728\u5934\u90e8\u521b\u4f24\u540e24\u5c0f\u65f6\u4e5f\u6ca1\u6709\u89c2\u5bdf\u5230CD31\u8840\u7ba1\u6807\u8bb0\u7269\u67d3\u8272\u7684\u660e\u663e\u5dee\u5f02\uff08\u56fe1\u2014\u9644\u56fe1b\u548cc\uff09\u3002\u6b64\u5916\uff0c\u6211\u4eec\u53ea\u5728\u9885\u8111\u635f\u4f24\u540e24\u5c0f\u65f6\u5728\u8111\u819c\u4e2d\u68c0\u6d4b\u5230\u8f7b\u5fae\u7684\u80f6\u8d28\u589e\u751f\uff08Iba1\u548cGFAP\u67d3\u8272\uff09\uff08\u56fe1\u2014\u9644\u56fe1d\uff0ce\u548cf\uff09\u4ee5\u53caMHCII +\u67d3\u8272\uff08\u56fe1\u2014\u9644\u56fe1g\uff0ch\u548ci\uff09\u3002\u5728\u672c\u6587\u7684\u6240\u6709\u6d4b\u5e8f\u7814\u7a76\u4e2d\uff0c\u6211\u4eec\u7b56\u7565\u6027\u5730\u9009\u62e9\u4ec5\u5206\u79bb\u80cc\u4fa7\u8111\u819c\u7ec4\u7ec7\uff0c\u56e0\u4e3a\u8fd9\u4e2a\u533a\u57df\u7684\u8111\u819c\u4e0d\u5305\u62ec\u76f4\u63a5\u635f\u4f24\u90e8\u4f4d\u7684\u7ec4\u7ec7\u3002\u56e0\u6b64\uff0c\u4ece\u8fd9\u4e9b\u7814\u7a76\u4ea7\u751f\u7684\u6d4b\u5e8f\u6570\u636e\u5e94\u66f4\u597d\u5730\u53cd\u6620\u7531\u5c40\u90e8\u635f\u4f24\u90e8\u4f4d\u5f15\u8d77\u7684\u5168\u5c40\u8111\u819c\u53d8\u5316\uff0c\u800c\u4e0d\u662f\u76f4\u63a5\u635f\u4f24\u90e8\u4f4d\u7684\u7ec4\u7ec7\u635f\u4f24\u548c\u54cd\u5e94\u3002\u5bf9Sham\u548cTBI\u8111\u819c\u7684\u8054\u5408\u805a\u7c7b\u663e\u793a\u4e8621\u4e2a\u72ec\u7279\u7684\u7ec6\u80de\u7fa4\uff0c\u5305\u62ec\u5185\u76ae\u7ec6\u80de\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de\u3001\u65bd\u4e07\u7ec6\u80de\u548c\u8111\u810a\u6db2\u8111\u5ba4\u4e0a\u76ae\u7ec6\u80de<\/strong>\uff08\u56fe1b\u548cc\uff0c\u88681\uff09\u3002\u6b64\u5916\uff0c\u8111\u819c\u5305\u542b\u5b8c\u6574\u7684\u514d\u75ab\u7ec6\u80de\u7ec4\u6210\uff0c\u5305\u62ec\u5de8\u566c\u7ec6\u80de\u3001B\u7ec6\u80de\u3001T\u7ec6\u80de\u3001NK\u7ec6\u80de\u3001\u6811\u7a81\u72b6\u7ec6\u80de\u3001\u6d46\u7ec6\u80de\u6837\u6811\u7a81\u72b6\u7ec6\u80de\u548c\u4e2d\u6027\u7c92\u7ec6\u80de\uff08\u56fe1b\u548cc\uff0c\u88681\uff09\u3002\u5176\u4ed6\u7ec6\u80de\u7fa4\u7684\u5b9a\u4e49\u8f83\u5dee\uff0c\u5305\u62ec\u8868\u8fbe\u4e0e\u51dd\u8840\u6709\u5173\u57fa\u56e0\u548c\u589e\u6b96\u7ec6\u80de\u7684\u7ec6\u80de\uff08\u56fe1b\u548cc\uff0c\u88681\uff09\u3002\u5728\u6309Sham\u548cTBI\u5904\u7406\u5206\u5f00\u65f6\uff0c\u8fd921\u4e2a\u7ec6\u80de\u7fa4\u5728\u4e24\u7ec4\u4e2d\u4ecd\u7136\u5b58\u5728<\/strong>\uff08\u56fe1d\uff0c\u88681\uff09\uff0c\u4f46\u9891\u7387\u6709\u6240\u4e0d\u540c\uff08\u56fe1e\uff09\u3002\u5728\u8111\u635f\u4f24\u540e\uff0c\u4e00\u7ec4\u5de8\u566c\u7ec6\u80de\u7684\u9891\u7387\u8f83\u9ad8\uff0c\u6211\u4eec\u5c06\u5176\u6807\u8bb0\u4e3a\u201c\u6d3b\u5316\u5de8\u566c\u7ec6\u80de1\u201d\uff0c\u56e0\u4e3a\u5b83\u4eec\u8868\u73b0\u51fa\u4e0e\u8865\u4f53\u76f8\u5173\u57fa\u56e0\u7684\u9ad8\u8868\u8fbe<\/strong>\uff08\u56fe1c\u548ce\uff09\u3002\u6b64\u5916\uff0c\u8111\u5916\u4f24\u540e\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u9891\u7387\u663e\u8457\u589e\u52a0\uff08\u56fe1e\uff09\u3002\u867d\u7136\u5176\u4ed6\u7ec6\u80de\u7c7b\u578b\u7684\u9891\u7387\u6709\u6240\u51cf\u5c11\uff0c\u5373B\u7ec6\u80de\u7fa4\uff0c\u4f46\u5c1a\u4e0d\u6e05\u695a\u8fd9\u662f\u76f8\u5bf9\u4e8e\u5176\u4ed6\u4e9a\u7fa4\u7684\u6269\u5f20\u8fd8\u662f\u5b9e\u9645\u6570\u91cf\u51cf\u5c11\uff08\u56fe1e\uff09\u3002\u4e3a\u786e\u4fdd\u6837\u54c1\u5236\u5907\u7684\u77ed\u6682\u6d88\u5316\u548c\u5904\u7406\u6b65\u9aa4\u6ca1\u6709\u5bfc\u81f4TBI\u548cSham\u6837\u672c\u4e2d\u5e94\u6fc0\u76f8\u5173\u57fa\u56e0\u7684\u663e\u8457\u4e0a\u8c03\uff0c\u6211\u4eec\u68c0\u67e5\u4e86\u4e00\u7ec4\u5df2\u77e5\u5728\u7ec4\u7ec7\u5904\u7406\u548c\u5e94\u6fc0\u6761\u4ef6\u4e0b\u4e0a\u8c03\u7684\u57fa\u56e0\uff08Haimon\u7b49\uff0c2018\u5e74; Marsh\u7b49\uff0c2020\u5e74; Van Hove\u7b49\uff0c2019\u5e74; \u56fe1\u2014\u9644\u56fe2\uff09\u3002\u6781\u5c11\u6570\u6d4b\u5e8f\u7684\u7ec6\u80de\u8868\u8fbe\u4e86\u8fd9\u4e9b\u57fa\u56e0\uff0c\u5e76\u4e14\u5728TBI\u6216Sham\u5b9e\u9a8c\u7ec4\u4e4b\u95f4\u6ca1\u6709\u5b9e\u8d28\u6027\u5dee\u5f02\uff0c\u8868\u660e\u5904\u7406\u5bf9\u57fa\u56e0\u8868\u8fbe\u7684\u8d21\u732e\u8f83\u5c0f\uff0c\u5e76\u4e14\u5728\u5b9e\u9a8c\u7ec4\u4e4b\u95f4\u6709\u76f8\u4f3c\u7684\u5f71\u54cd\uff08\u56fe1\u2014\u9644\u56fe2\uff09\u3002\u603b\u4f53\u800c\u8a00\uff0c\u8fd9\u4e9b\u6570\u636e\u7a81\u663e\u4e86\u8111\u819c\u7ec4\u7ec7\u7684\u5f02\u8d28\u6027\uff0c\u5e76\u4e14\u8bc1\u660e\u4e86\u5de8\u566c\u7ec6\u80de\u548c\u6210\u7ea4\u7ef4\u7ec6\u80de\u7fa4\u7684\u9891\u7387\u5728TBI\u540e1\u5468\u589e\u52a0\u3002<\/p>\n\n\n\n

\"\u56fe\u7247[1]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n
\"\u56fe\u7247[2]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n
\"\u56fe\u7247[3]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

2.2 \u8f7b\u5ea6\u8111\u5916\u4f24\u5bf9\u8111\u819c\u5de8\u566c\u7ec6\u80de\u8f6c\u5f55\u7ec4\u7684\u5f71\u54cd<\/h2>\n\n\n\n

\u9274\u4e8e\u6211\u4eec\u7684\u6570\u636e\u663e\u793a\u4f24\u540e\u8111\u819c\u5de8\u566c\u7ec6\u80de\u7fa4\u4f53\u660e\u663e\u6269\u5f20\uff08\u56fe1e\uff09\uff0c\u4ee5\u53ca\u65b0\u5174\u6570\u636e\u8868\u660e\u8fd9\u4e9b\u7ec6\u80de\u5728\u521b\u4f24\u6027\u8111\u635f\u4f24\uff08TBI\uff09\u75c5\u7406\u53d1\u751f\u4e2d\u626e\u6f14\u91cd\u8981\u89d2\u8272\uff08Russo\u7b49\uff0c2018\u5e74\uff1bRoth\u7b49\uff0c2014\u5e74\uff09\uff0c\u6211\u4eec\u51b3\u5b9a\u9996\u5148\u5173\u6ce8\u8111\u819c\u5de8\u566c\u7ec6\u80de\u5bf9\u5934\u90e8\u521b\u4f24\u7684\u53cd\u5e94\u3002\u5bf9\u201c\u6fc0\u6d3b\u7684\u5de8\u566c\u7ec6\u80de\u201d\u7fa4\u4f53\uff08\u6fc0\u6d3b\u7684\u5de8\u566c\u7ec6\u80de1&2\uff09\u7684\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u663e\u793a\uff0c\u5728\u5934\u90e8\u53d7\u4f24\u540e\uff0c\u6709321\u4e2a\u4e0a\u8c03\u57fa\u56e0\u548c369\u4e2a\u4e0b\u8c03\u57fa\u56e0\uff0c\u4f7f\u7528\u7684\u9519\u8bef\u53d1\u73b0\u7387<0.1\uff08\u56fe2a\uff09\u3002\u5f53\u6211\u4eec\u5bf9\u8fd9\u4e9b\u7fa4\u4f53\u4e2d\u663e\u8457\u4e0a\u8c03\u7684\u57fa\u56e0\u8fdb\u884c\u7f51\u7edc\u5206\u6790\u65f6\uff0c\u6211\u4eec\u53d1\u73b0\u4e0e\u514d\u75ab\u7cfb\u7edf\u6fc0\u6d3b\u76f8\u5173\u7684\u9014\u5f84\u5bcc\u96c6\uff08\u56fe2b\uff09\u3002\u6fc0\u6d3b\u7fa4\u4f53\u4e2d\u4e0a\u8c03\u7684\u57fa\u56e0\u5305\u62ec\u90a3\u4e9b\u5bf9\u7ec6\u80de\u56e0\u5b50\u5206\u6ccc\u3001\u514d\u75ab\u7ec6\u80de\u5206\u5316\u3001\u8fd0\u52a8\u6027\u548c\u8d8b\u5316\u6027\u91cd\u8981\u7684\u57fa\u56e0\uff08\u56fe2b\uff09\u3002\u6b64\u5916\uff0c\u5bf9\u5934\u90e8\u521b\u4f24\u7684\u53cd\u5e94\u4e2d\u6700\u9ad8\u5bcc\u96c6\u7684\u57fa\u56e0\u672c\u4f53\uff08GO\uff09\u751f\u7269\u8fc7\u7a0b\u88ab\u53d1\u73b0\u4e0e\u514d\u75ab\u7cfb\u7edf\u6fc0\u6d3b\u548c\u5e94\u6fc0\u53cd\u5e94\u76f8\u5173\uff08\u56fe2c\uff09\u3002\u6211\u4eec\u8fd8\u6ce8\u610f\u5230\uff0c\u5bf9\u514d\u75ab\u76f8\u5173GO\u672f\u8bed\u8d21\u732e\u6700\u663e\u8457\u7684\u4e0a\u8c03\u57fa\u56e0\u4e2d\uff0c\u6709\u4e9b\u5bf9I\u578b\u5e72\u6270\u7d20\uff08IFN\uff09\u53cd\u5e94\u5f88\u91cd\u8981\uff0c\u5305\u62ecIfnar1\u3001Ifi203\u3001Irf2bp2\u548cIrf5\u7b49\uff08\u56fe2d\uff09\u3002\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u901a\u8fc7qPCR\u68c0\u67e5\u4e86\u4f24\u540e\u4e00\u5468\u6574\u4e2a\u8111\u819c\u6837\u672c\u4e2dI\u578bIFN\u57fa\u56e0\u8868\u8fbe\u3002\u6211\u4eec\u89c2\u5bdf\u5230\u5305\u62ecIfnar1\u3001Irf5\u3001Ifnb1\u548cIfi203\u5728\u5185\u7684\u57fa\u56e0\u8868\u8fbe\u5347\u9ad8\uff0c\u51f8\u663e\u4e86TBI\u540e\u5f3a\u70c8\u7684I\u578bIFN\u57fa\u56e0\u7279\u5f81\uff0c\u8fd9\u53ef\u80fd\u662f\u7531\u8111\u819c\u5de8\u566c\u7ec6\u80de\u9a71\u52a8\u7684<\/strong>\uff08\u56fe2e\u2013h\uff09\u3002\u6709\u8da3\u7684\u662f\uff0c\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660e\uff0c\u5927\u8111\u5b9e\u8d28\u4e2dI\u578bIFN\u4fe1\u53f7\u7684\u5347\u9ad8\u662fTBI\u75c5\u7406\u53d1\u751f\u4e2d\u4e0d\u826f\u7ed3\u679c\u7684\u9a71\u52a8\u56e0\u7d20\uff08Karve\u7b49\uff0c2016\u5e74\uff1bBarrett\u7b49\uff0c2020\u5e74\uff09\u3002<\/p>\n\n\n\n

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\"\u56fe\u7247[4]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n
\"\u56fe\u7247[5]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

<\/h2>\n\n\n\n

2.3 \u8f7b\u5ea6TBI\u8bf1\u5bfc\u8111\u819c\u6210\u7ea4\u7ef4\u7ec6\u80de\u4e2d\u4e0e\u795e\u7ecf\u75be\u75c5\u76f8\u5173\u57fa\u56e0\u7684\u4e0a\u8c03<\/h2>\n\n\n\n

\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u60f3\u8fdb\u4e00\u6b65\u7814\u7a76\u201c\u6210\u7ea4\u7ef4\u7ec6\u80de\u201d\u7fa4\u4f53\uff0c\u56e0\u4e3a\u5728\u5355\u7ec6\u80de\u6570\u636e\u96c6\u4e2d\u663e\u793a\uff0c\u4f24\u540e1\u5468\u8be5\u7fa4\u4f53\u6709\u6240\u6269\u5f20\uff08\u56fe1d\u548ce\uff09\u3002\u5934\u90e8\u53d7\u4f24\u540e\uff0c\u201c\u6210\u7ea4\u7ef4\u7ec6\u80de\u201d\u7fa4\u4f53\u5c55\u73b0\u51fa368\u4e2a\u6fc0\u6d3b\u57fa\u56e0\u548c320\u4e2a\u6291\u5236\u57fa\u56e0\uff08\u56fe3a\uff09\u3002\u4f24\u540e\u6fc0\u6d3b\u57fa\u56e0\u4e2d\u6709\u51e0\u79cd\u6a21\u5f0f\uff0c\u5305\u62ec\u5bf9\u80f6\u539f\u86cb\u767d\u751f\u4ea7\u548c\u7ec6\u80de\u5916\u57fa\u8d28\u91cd\u5851\u91cd\u8981\u7684\u57fa\u56e0\uff08\u5982Nisch\u3001Ppib\u3001Pmepa1\u3001Ddr2\uff09\u548c\u5bf9\u7ec6\u80de\u8fd0\u52a8\u6027\u548c\u751f\u957f\u81f3\u5173\u91cd\u8981\u7684\u57fa\u56e0\uff08\u5982Ptprs\u3001Pfn1\u3001Cd302\u3001Tpm3\uff09\uff08\u56fe3b\uff09\u3002\u4e3a\u4e86\u9a8c\u8bc1\u8fd9\u4e9b\u6d4b\u5e8f\u53d1\u73b0\uff0c\u6211\u4eec\u5229\u7528\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\u67d3\u8272\u65b9\u6cd5\u5bf9\u6210\u7ea4\u7ef4\u7ec6\u80de\u4ea7\u751f\u7684\u80f6\u539f\u86cb\u767d\u8fdb\u884c\u4e86\u67d3\u8272\uff0c\u8fd9\u5728\u8111\u819c\u6574\u4f53\u88c5\u7247\u4e2d\u8fdb\u884c\u3002\u4e0e\u6d4b\u5e8f\u7ed3\u679c\u4e00\u81f4\uff0c\u6211\u4eec\u89c2\u5bdf\u5230\u5728TBI\u540e1\u5468\u80f6\u539f\u86cb\u767d\u5bc6\u5ea6\u663e\u8457\u589e\u52a0\uff08\u56fe3c\u3001d\u548ce\uff09\u3002\u6b64\u5916\uff0c\u6211\u4eec\u8fd8\u6709\u5174\u8da3\u786e\u5b9a\u6210\u7ea4\u7ef4\u7ec6\u80de\u7fa4\u4f53\u662f\u5426\u5bf9TBI\u540e\u7684\u708e\u75c7\u53cd\u5e94\u6709\u6240\u8d21\u732e\u3002\u5728TBI\u540e\u6210\u7ea4\u7ef4\u7ec6\u80de\u4e2d\u8bc6\u522b\u51fa\u7684\u663e\u8457\u4e0a\u8c03\u57fa\u56e0\u4e2d\uff0c\u8bb8\u591a\u4e0e\u514d\u75ab\u7cfb\u7edf\u6fc0\u6d3b\u548c\u7ec6\u80de\u56e0\u5b50\u4fe1\u53f7\u4f20\u5bfc\u7684\u7ec4\u5206\u76f8\u5173\uff08\u56fe3f\uff09\u3002<\/p>\n\n\n\n

\u4e3a\u4e86\u63a2\u7d22\u8f7b\u5ea6\u5934\u90e8\u521b\u4f24\u540e\u6210\u7ea4\u7ef4\u7ec6\u80de\u6700\u53d7\u5f71\u54cd\u7684\u7ec6\u80de\u548c\u75be\u75c5\u9014\u5f84\uff0c\u6211\u4eec\u9274\u5b9a\u4e86\u4e0eTBI\u540e\u4e0e\u5047\u624b\u672f\u7ec4\u76f8\u6bd4\u5728\u6210\u7ea4\u7ef4\u7ec6\u80de\u7fa4\u4f53\u4e2d\u5dee\u5f02\u6027\u4e0a\u8c03\u7684\u57fa\u56e0\u5bcc\u96c6\u7684<\/p>\n\n\n\n

KEGG\u672f\u8bed\u3002\u6709\u8da3\u7684\u662f\uff0c\u4e0e\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u76f8\u5173\u7684\u75be\u75c5\u9014\u5f84\uff0c\u5305\u62ec\u5e15\u91d1\u68ee\u75c5\u3001\u963f\u5c14\u8328\u6d77\u9ed8\u75c5\u3001\u808c\u840e\u7f29\u4fa7\u7d22\u786c\u5316\u75c7\u548c\u670a\u75c5\u6bd2\u75c5\uff0c\u662fTBI\u540e\u5728\u6210\u7ea4\u7ef4\u7ec6\u80de\u4e2d\u4e00\u4e9b\u6700\u9ad8\u4e0a\u8c03\u7684\u9014\u5f84\uff08\u56fe3g\uff09\u3002\u8bb8\u591a\u4e0e\u6c27\u5316\u78f7\u9178\u5316KEGG\u672f\u8bed\u6709\u8d21\u732e\u7684\u76f8\u540c\u672f\u8bed\u4e5f\u6709\u52a9\u4e8e\u5404\u79cd\u75be\u75c5\u76f8\u5173\u7684KEGG\u672f\u8bed\uff0c\u8868\u660e\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u4ee3\u8c22\u72b6\u6001\u53d8\u5316\u53ef\u80fd\u662f\u75be\u75c5\u76f8\u5173\u8fc7\u7a0b\u7684\u57fa\u7840\u3002<\/p>\n\n\n\n

\u9274\u4e8e\u6210\u7ea4\u7ef4\u7ec6\u80de\u5b58\u5728\u4e8e\u6240\u6709\u4e09\u5c42\u8111\u819c\u4e2d\uff08DeSisto\u7b49\uff0c2020\u5e74\uff09\uff0c\u6211\u4eec\u51b3\u5b9a\u8c03\u67e5\u6210\u7ea4\u7ef4\u7ec6\u80de\u6240\u5728\u7684\u5c42\u6b21\uff0c\u4ee5\u53ca\u54ea\u4e00\u5c42\u53ef\u80fd\u5bf9TBI\u540e\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u589e\u52a0\u8d1f\u8d23\u3002\u4e3a\u6b64\uff0c\u6211\u4eec\u68c0\u67e5\u4e86\u901a\u5e38\u7528\u4e8e\u8bc6\u522b\u6210\u7ea4\u7ef4\u7ec6\u80de\u53ef\u80fd\u5c45\u4f4f\u7684\u8111\u819c\u4e0d\u540c\u5c42\u6b21\u7684\u5206\u5b50\u8868\u8fbe\uff08DeSisto\u7b49\uff0c2020\u5e74\uff1bDoro\u7b49\uff0c2019\u5e74\uff1bCooper\u7b49\uff0c2012\u5e74\uff1bZarbalis\u7b49\uff0c2007\u5e74\uff1bKalamarides\u7b49\uff0c2011\u5e74\uff1bSiegenthaler\u7b49\uff0c2009\u5e74\uff1bCaglayan\u7b49\uff0c2014\u5e74\uff09\u3002\u66f4\u5177\u4f53\u5730\u8bf4\uff0c\u8fd1\u671f\u7814\u7a76\u8868\u660e\uff0c\u786c\u819c\u6210\u7ea4\u7ef4\u7ec6\u80de\u53ef\u4ee5\u4f7f\u7528Alpl\u548cFoxc2\u6807\u8bb0\u7269\u6765\u8bc6\u522b\uff08Doro\u7b49\uff0c2019\u5e74\uff1bCooper\u7b49\uff0c2012\u5e74\uff1bZarbalis\u7b49\uff0c2007\u5e74\uff09\uff0c\u800cPtgds\u548cAld1a2\u662f\u86db\u7f51\u819c\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u72ec\u7279\u6807\u8bb0\u7269\uff08Kalamarides\u7b49\uff0c2011\u5e74\uff1bSiegenthaler\u7b49\uff0c2009\u5e74\uff09\uff0cCol18a1\u5219\u7279\u5f02\u4e8e\u8111\u819c\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08DeSisto\u7b49\uff0c2020\u5e74\uff1bCaglayan\u7b49\uff0c2014\u5e74\uff09\u3002\u4e0d\u51fa\u6240\u6599\uff0c\u6211\u4eec\u53d1\u73b0\u8111\u819c\u4e2d\u7684\u5927\u591a\u6570\u6210\u7ea4\u7ef4\u7ec6\u80de\u6765\u81ea\u786c\u819c\uff0c\u8fd9\u662f\u8111\u819c\u4e2d\u6700\u539a\u7684\u5c42\uff0c\u4e5f\u662f\u8fd9\u4e9b\u7814\u7a76\u4e2d\u6240\u4f7f\u7528\u7684\u89e3\u5256\u65b9\u6cd5\u6240\u9488\u5bf9\u7684\u5c42\u6b21\uff08Alves de Lima\u7b49\uff0c2020b\uff1bRua\u548cMcGavern\uff0c2018\u5e74\uff09\u3002\u5982\u9884\u671f\uff0c\u5c11\u91cf\u7684\u8111\u819c\u6216\u86db\u7f51\u819c\u5c45\u4f4f\u6210\u7ea4\u7ef4\u7ec6\u80de\u51fa\u73b0\uff08\u56fe3h\uff09\u3002\u5f53\u6211\u4eec\u89c2\u5bdfTBI\u524d\u540e\u786c\u819c\u6210\u7ea4\u7ef4\u7ec6\u80de\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u65f6\uff0c\u6211\u4eec\u53d1\u73b0\u4e00\u4e9b\u6807\u8bb0\u7269\uff08\u4f8b\u5982Foxc2\uff0cFxyd5\uff09\u5728\u53d7\u4f24\u540e\u663e\u8457\u4e0a\u8c03\uff0c\u800c\u5176\u4ed6\u786c\u819c\u6807\u8bb0\u7269\u867d\u7136\u6ca1\u6709\u5728\u663e\u8457\u66f4\u9ad8\u6c34\u5e73\u4e0a\u8868\u8fbe\uff0c\u4f46\u663e\u7136\u7531\u66f4\u9ad8\u6bd4\u4f8b\u7684\u603b\u7ec6\u80de\u8868\u8fbe\uff08\u4f8b\u5982Alpl\uff0cMgp\uff09\uff08\u56fe3i\uff09\u3002\u8fd9\u8868\u660e\u5728\u8111\u635f\u4f24\u540e\u4e00\u5468\uff0c\u786c\u819c\u90e8\u4f4d\u53ef\u80fd\u7ecf\u5386\u4e86\u6210\u7ea4\u7ef4\u7ec6\u80de\u9891\u7387\u7684\u589e\u52a0\uff0c\u8fd9\u4e0eTBI\u540e\u4e00\u5468\u5728\u8111\u819c\u4e2d\u770b\u5230\u7684\u80f6\u539f\u86cb\u767d\u5bc6\u5ea6\u589e\u52a0\u4e5f\u662f\u4e00\u81f4\u7684\uff08\u56fe3c\uff0cd\u548ce\uff09\u3002\u603b\u7684\u6765\u8bf4\uff0c\u6211\u4eec\u89c2\u5bdf\u5230\u8111\u819c\u6210\u7ea4\u7ef4\u7ec6\u80de\u7fa4\u4f53\u5bf9TBI\u6709\u9ad8\u5ea6\u7684\u54cd\u5e94\u6027\uff0c\u5b83\u4eec\u4e0a\u8c03\u4e86\u5bcc\u542b\u75be\u75c5\u76f8\u5173\u9014\u5f84\u3001\u514d\u75ab\u7cfb\u7edf\u6fc0\u6d3b\u548c\u4f24\u53e3\u6108\u5408\u53cd\u5e94\u7684\u57fa\u56e0\u3002<\/p>\n\n\n\n

\"\u56fe\u7247[6]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n
\"\u56fe\u7247[7]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

2.4 \u8f7b\u5ea6TBI\u5bf9\u8111\u819c\u6dcb\u5df4\u7ec6\u80de\u7684\u8f6c\u5f55\u8c03\u8282<\/h2>\n\n\n\n

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\"\u56fe\u7247[9]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

2.5 \u9884\u6d4b\u7684\u914d\u4f53-\u9776\u6807\u76f8\u4e92\u4f5c\u7528\u51f8\u663e\u4e86TBI\u540e\u4fc3\u751f\u957f\u548c\u53d7\u63a7\u5236\u7684\u4fc3\u708e\u75c7\u8111\u819c\u73af\u5883<\/h2>\n\n\n\n

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2.6 \u8870\u8001\u548c\u8f7b\u5ea6\u8111\u5916\u4f24\u5bf9\u8111\u819c\u8f6c\u5f55\u7ec4\u7684\u663e\u8457\u5f71\u54cd<\/h2>\n\n\n\n

\u5728\u5e74\u8f7b\u5c0f\u9f20\u4e2d\u89c2\u5bdf\u5230\u7684\u8111\u635f\u4f24\u5f15\u8d77\u7684\u8111\u819c\u8f6c\u5f55\u548c\u7ec6\u80de\u666f\u89c2\u7684\u663e\u8457\u53d8\u5316\u540e\uff0c\u6211\u4eec\u63a5\u4e0b\u6765\u6709\u5174\u8da3\u8c03\u67e5\u8fd9\u4e9b\u53d8\u5316\u662f\u5426\u5728\u8001\u5e74\u4eba\u4e2d\u4fdd\u6301\u4e0d\u53d8\u6216\u53d1\u751f\u6539\u53d8\u3002\u5148\u524d\u66fe\u6709\u4eba\u63d0\u51fa\uff0c\u5934\u90e8\u521b\u4f24\u540e\u5927\u8111\u65e0\u6cd5\u6709\u6548\u89e3\u51b3\u708e\u75c7\u53cd\u5e94\uff0c\u8fd9\u5bfc\u81f4\u8001\u5e74\u4eba\u4e2d\u5e38\u89c1\u7684\u75be\u75c5\u8fdb\u7a0b\u52a0\u91cd\uff08Chou et al., 2018; Witcher et al., 2021; Witcher et al., 2018\uff09\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u7279\u522b\u6709\u5174\u8da3\u63a2\u7d22\u5e74\u8f7b\u548c\u8001\u5e74\u5c0f\u9f20\u4e4b\u95f4\u5728TBI\u540e\u8111\u819c\u514d\u75ab\u53cd\u5e94\u89e3\u51b3\u65b9\u9762\u7684\u6f5c\u5728\u5dee\u5f02\u3002\u4e3a\u6b64\uff0c\u6211\u4eec\u5728\u5e74\u8f7b\u5c0f\u9f20\uff0810\u5468\u9f84\uff09\u548c\u8001\u5e74\u5c0f\u9f20\uff0820\u4e2a\u6708\u9f84\uff09\u4e2d\uff0c\u5747\u5728TBI\u6216Sham\u5904\u7406\u540e1.5\u4e2a\u6708\u8fdb\u884c\u4e86\u8111\u819c\u7ec4\u7ec7\u7684\u6279\u91cfRNA\u6d4b\u5e8f\uff0c\u56e0\u4e3a\u6211\u4eec\u9884\u6d4b\u5e74\u8f7b\u5c0f\u9f20\u4e2d\u7684\u8111\u819c\u635f\u4f24\u5c06\u5728TBI\u540e1.5\u4e2a\u6708\u5927\u90e8\u5206\u5f97\u5230\u89e3\u51b3\uff08\u56fe5a\uff09\u3002\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u663e\u793a\u5e74\u9f84\u662f\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe\u7684\u4e3b\u8981\u9a71\u52a8\u56e0\u7d20\uff0c\u56e0\u4e3a\u5e74\u8f7b\u7ec4\u548c\u8001\u5e74\u7ec4\u805a\u7c7b\u6700\u8fdc<\/strong>\uff08\u56fe5b\uff09\u3002\u7136\u800c\uff0c\u5c3d\u7ba1\u5e74\u8f7b\u5c0f\u9f20\u63a5\u53d7Sham\u6216TBI\u7684\u7ec4\u805a\u96c6\u5728PCA\u56fe\u4e2d\uff0c\u8001\u5e74Sham\u548cTBI\u5c0f\u9f20\u7684\u7ec4\u805a\u7c7b\u66f4\u5206\u6563\uff0c\u8868\u660e\u4e0e\u5e74\u8f7b\u5c0f\u9f20\u76f8\u6bd4\uff0c\u8001\u5e74\u5c0f\u9f20\u7684\u57fa\u56e0\u8868\u8fbe\u53d7TBI\u7684\u5f71\u54cd\u53ef\u80fd\u66f4\u6301\u4e45\uff08\u56fe5b\uff09\u3002\u5b9e\u9645\u4e0a\uff0c\u5f53\u6211\u4eec\u89c2\u5bdf\u4e86\u6240\u6709\u56db\u4e2a\u5b9e\u9a8c\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u6570\u91cf\u65f6\uff0c\u6211\u4eec\u53d1\u73b0\u5e74\u8f7bSham\u4e0e\u5e74\u8f7bTBI\u4e4b\u95f4\u4ec5\u670922\u4e2a\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff0c\u800c\u8001\u5e74Sham\u4e0e\u8001\u5e74TBI\u4e4b\u95f4\u6709364\u4e2a\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08\u56fe5c\u548cd\uff09\u3002\u6709\u8da3\u7684\u662f\uff0c\u5728\u6bd4\u8f83\u5e74\u8f7bSham\u5c0f\u9f20\u4e0e\u8001\u5e74Sham\u5c0f\u9f20\u65f6\uff0c\u53d1\u73b0\u4e861772\u4e2a\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff0c\u800c\u5728\u6bd4\u8f83\u5e74\u8f7bTBI\u5c0f\u9f20\u4e0e\u8001\u5e74TBI\u5c0f\u9f20\u65f6\uff0c\u53d1\u73b0\u4e862936\u4e2a\u5dee\u5f02\u8c03\u63a7\u57fa\u56e0\uff08\u56fe5c\u548cd\uff09\u3002\u6b64\u5916\uff0c\u6211\u4eec\u8fd8\u67e5\u770b\u4e86\u54ea\u4e9b\u57fa\u56e0\u5728\u6bd4\u8f83\u7ec4\u4e4b\u95f4\u5171\u4eab\uff0c\u4ee5\u786e\u5b9a\u8001\u5e74TBI\u4e2d\u7684TBI\u7279\u5f81\u662f\u5426\u72ec\u7279\u6216\u4e3b\u8981\u4e0e\u672a\u53d7\u4f24\u7684\u8001\u5e74\u5c0f\u9f20\u5171\u4eab\u3002\u6709\u8da3\u7684\u662f\uff0c\u6709917\u4e2a\u4e0b\u8c03\u57fa\u56e0\u548c639\u4e2a\u4e0a\u8c03\u57fa\u56e0\u662f\u5e74\u8f7bTBI\u4e0e\u8001\u5e74TBI\u4e4b\u95f4\u72ec\u6709\u7684\uff0c\u4e0e\u4efb\u4f55\u5176\u4ed6\u6bd4\u8f83\uff0c\u5305\u62ec\u5e74\u8f7bSham\u4e0e\u8001\u5e74Sham\u7684\u6bd4\u8f83\u90fd\u4e0d\u5171\u4eab\u3002\u6709595\u4e2a\u4e0b\u8c03\u57fa\u56e0\u548c592\u4e2a\u4e0a\u8c03\u57fa\u56e0\u5728\u8fd9\u4e24\u4e2a\u6bd4\u8f83\u4e4b\u95f4\u5171\u4eab\uff0c\u8fd9\u8868\u660e\u5c3d\u7ba1\u90e8\u5206\u5728\u5e74\u8f7bTBI\u4e0e\u8001\u5e74TBI\u7ec4\u4e2d\u770b\u5230\u7684\u8f6c\u5f55\u53d8\u5316\u53ef\u80fd\u5f52\u56e0\u4e8e\u8870\u8001\uff0c\u4f46\u5728\u53d7\u4f24\u8001\u5e74\u5c0f\u9f20\u4e2d\u6709\u5927\u91cf\u53d7\u5f71\u54cd\u7684\u57fa\u56e0\u662f\u5728\u5916\u4f24\u60c5\u51b5\u4e0b\u5728\u8001\u5e74\u5c0f\u9f20\u4e2d\u72ec\u7279\u8bc6\u522b\u7684\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u8868\u660e\u8001\u5316\u6df1\u523b\u5f71\u54cd\u4e86\u8111\u819c\u57fa\u56e0\u8868\u8fbe\uff0c\u5e76\u4e14\u8001\u5e74\u4eba\u5728\u7ecf\u5386\u8f7b\u5ea6\u5934\u90e8\u521b\u4f24\u540e\u57fa\u56e0\u8868\u8fbe\u53d1\u751f\u4e86\u66f4\u5927\u7684\u53d8\u5316\u3002\u6b64\u5916\uff0c\u867d\u7136\u5e74\u8f7b\u5c0f\u9f20\u5728TBI\u540e1.5\u4e2a\u6708\u51e0\u4e4e\u6ca1\u6709\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\uff0c\u4f46\u8001\u5e74\u5c0f\u9f20\u5728\u66f4\u957f\u7684\u65f6\u95f4\u5185\u7ecf\u5386\u4e86\u66f4\u591a\u7684\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\uff0c\u8fd9\u8868\u660e\u8001\u5e74\u4eba\u5728TBI\u540e\u7684\u6062\u590d\u53ef\u80fd\u4f1a\u53d7\u5230\u5ef6\u8fdf\u3002<\/p>\n\n\n\n

\u7531\u4e8e\u8001\u5316\u672c\u8eab\u5bfc\u81f4\u4e86\u660e\u663e\u4e0d\u540c\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\uff0c\u6211\u4eec\u51b3\u5b9a\u5728\u6279\u91cfRNA-seq\u6570\u636e\u96c6\u4e2d\u66f4\u4ed4\u7ec6\u5730\u7814\u7a76\u8fd9\u4e9b\u5dee\u5f02\u3002\u5728\u68c0\u67e5\u8001\u5e74Sham\u5c0f\u9f20\u4e0e\u5e74\u8f7bSham\u5c0f\u9f20\u76f8\u6bd4\u524d20\u4e2a\u6700\u663e\u8457\u4e0a\u8c03\u548c\u4e0b\u8c03\u7684\u57fa\u56e0\u65f6\uff0c\u6211\u4eec\u6ce8\u610f\u5230\u4e0eB\u7ec6\u80de\u6297\u4f53\u4ea7\u751f\u76f8\u5173\u7684\u57fa\u56e0\u660e\u663e\u4e0a\u8c03\uff08\u56fe6a\uff09\u3002\u4e8b\u5b9e\u4e0a\uff0c\u524d20\u4e2a\u4e0a\u8c03\u57fa\u56e0\u4e2d\u6709\u4e00\u534a\u5c5e\u4e8e\u8fd9\u4e00\u7c7b\u522b\uff08\u56fe6a\uff09\u3002\u5f53\u6211\u4eec\u68c0\u67e5\u4e86\u5e74\u8f7bSham\u5c0f\u9f20\u4e0e\u8001\u5e74Sham\u5c0f\u9f20\u6bd4\u8f83\u4e2d\u663e\u8457\u6fc0\u6d3b\u7684\u57fa\u56e0\u6240\u5bcc\u96c6\u7684\u524d20\u4e2aGO\u751f\u7269\u8fc7\u7a0b\u65f6\uff0c\u6211\u4eec\u53d1\u73b0\u514d\u75ab\u548c\u9632\u5fa1\u53cd\u5e94\u4f4d\u5c45\u5176\u4e2d\uff08\u56fe6b\uff09\uff0c\u8868\u660e\u8001\u5e74\u8111\u819c\u5185\u7684\u7ec6\u80de\u5df2\u7ecf\u5728\u7a33\u6001\u6761\u4ef6\u4e0b\u663e\u8457\u4e0a\u8c03\u4e86\u5b83\u4eec\u7684\u514d\u75ab\u53cd\u5e94\u3002\u7531\u4e8e\u4e0e\u6297\u4f53\u76f8\u5173\u57fa\u56e0\u7684\u663e\u8457\u4e0a\u8c03\u975e\u5e38\u660e\u663e\uff0c\u5e76\u4e14\u6700\u8fd1\u7684\u7814\u7a76\u62a5\u544a\u79f0\uff0c\u968f\u7740\u5e74\u9f84\u7684\u589e\u957f\uff0cIgA\u5206\u6ccc\u6d46\u7ec6\u80de\u589e\u52a0\uff08Fitzpatrick et al., 2020\uff09\uff0c\u6211\u4eec\u5728\u6279\u91cfRNA-seq\u6570\u636e\u96c6\u4e2d\u66f4\u4ed4\u7ec6\u5730\u68c0\u67e5\u4e86\u5176\u4e2d\u4e00\u4e9b\u57fa\u56e0\uff08\u56fe6c\uff09\u3002\u6211\u4eec\u53d1\u73b0\u4e0e\u514d\u75ab\u7403\u86cb\u767d\u91cd\u94fe\uff08Ighm\u3001Ighg2b\u3001Igha\uff09\u3001\u8f7b\u94fe\uff08Igkc\uff09\u4ee5\u53caIgA\u6216IgM\u6297\u4f53\u7684\u7ec4\u5206\u76f8\u5173\u7684\u57fa\u56e0\u663e\u8457\u4e0a\u8c03\uff08\u56fe6c\uff09\u3002<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u4f5c\u4e3a\u7b2c\u4e8c\u79cd\u9a8c\u8bc1\u65b9\u6cd5\uff0c\u6211\u4eec\u5bf9\u6574\u4e2a\u786c\u819c\u8111\u819c\u8fdb\u884c\u4e86\u6d41\u5f0f\u7ec6\u80de\u672f\uff0c\u4ee5\u8bc4\u4f30B220+\u548cCD19+\u7ec6\u80de\u5728\u603bCD45+\u7ec6\u80de\u4e2d\u7684\u6570\u91cf\u548c\u9891\u7387\uff08\u56fe6\u2014\u56fe\u8868\u8865\u51451a\uff09\u3002\u4e0e\u5e74\u8f7b\u7684\u786c\u819c\u8111\u819c\u76f8\u6bd4\uff0c\u8001\u5e74\u786c\u819c\u8111\u819c\u7684\u7ec6\u80de\u603b\u6570\u8f83\u5c11\uff08\u56fe6\u2014\u56fe\u8868\u8865\u51451b\uff09\u3002\u5728\u68c0\u67e5B220+\u3001CD19+\u548cB220+CD19+\u7ec6\u80de\u603b\u6570\u65f6\uff0c\u6211\u4eec\u5728\u8001\u5e74\u5c0f\u9f20\u4e2d\u6ca1\u6709\u770b\u5230\u663e\u8457\u5dee\u5f02\uff08\u56fe6\u2014\u56fe\u8868\u8865\u51451c\uff09\u3002\u867d\u7136\u4e0d\u663e\u8457\uff0c\u4f46\u4e0e\u5e74\u8f7b\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u8001\u5e74\u786c\u819c\u8111\u819c\u4e2dB220+\u3001CD19+\u548cB220+CD19+\u7ec6\u80de\u7684\u6574\u4f53\u9891\u7387\u6709\u6240\u4e0b\u964d\uff08\u56fe6\u2014\u56fe\u8868\u8865\u51451d\uff09\u3002\u603b\u7684\u6765\u8bf4\uff0c\u6211\u4eec\u6ca1\u6709\u5728\u8001\u5e74\u786c\u819c\u4e2d\u770b\u5230B\u7ec6\u80de\u6570\u91cf\u7684\u589e\u52a0\uff0c\u8fd9\u8868\u660e\u5728\u8001\u9f84\u5316\u8fc7\u7a0b\u4e2d\u89c2\u5bdf\u5230\u7684\u4e0e\u6297\u4f53\u76f8\u5173\u7684\u57fa\u56e0\u8868\u8fbe\u7684\u589e\u52a0\u53ef\u80fd\u53cd\u6620\u4e86\u8111\u819c\u4e2dB\u7ec6\u80de\u529f\u80fd\u7684\u53d8\u5316\uff0c\u800c\u4e0d\u662f\u66f4\u591aB\u7ec6\u80de\u7684\u62db\u52df\u3002\u6211\u4eec\u7684\u6570\u636e\u4e0e\u5176\u4ed6\u5df2\u53d1\u8868\u7814\u7a76\u7ed3\u679c\u7684\u5dee\u5f02\u53ef\u80fd\u53cd\u6620\u4e86\u7531\u4e8e\u8001\u5e74\u5c0f\u9f20\u8111\u819c\u6dcb\u5df4\u7ba1\u53d7\u635f\uff0c\u6cbf\u6a2a\u7aa6\u7684B\u7ec6\u80de\u7fa4\u4f53\u5728\u533a\u57df\u4e0a\u7684\u5dee\u5f02\u3002\u6211\u4eec\u72ec\u7279\u53d1\u73b0\u7684\u5176\u4ed6\u6f5c\u5728\u9a71\u52a8\u56e0\u7d20\u5305\u62ec\u6027\u522b\u548c\u5fae\u751f\u7269\u7fa4\u7684\u5dee\u5f02\uff0c\u8fd9\u4e24\u8005\u4e5f\u53ef\u80fd\u5f71\u54cd\u8111\u819c\u4e2dB\u7ec6\u80de\u7684\u6570\u91cf\u548c\u9891\u7387\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u8868\u660e\u8001\u5e74\u5c0f\u9f20\u4e2d\u7684B\u7ec6\u80de\u7fa4\u4f53\u7ec4\u6210\u53ef\u80fd\u4e0e\u5e74\u8f7b\u5c0f\u9f20\u6709\u663e\u8457\u4e0d\u540c\uff1b\u7136\u800c\uff0c\u672a\u6765\u7684\u7814\u7a76\u9700\u8981\u66f4\u8be6\u7ec6\u5730\u6b63\u5f0f\u8bc4\u4f30\u8fd9\u4e00\u70b9\u3002<\/p>\n\n\n\n

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\"\u56fe\u7247[10]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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\"\u56fe\u7247[11]-\u5403\u4e86\u5403\u4e86\"<\/figure>\n\n\n\n

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