{"id":515,"date":"2023-09-09T00:35:30","date_gmt":"2023-09-08T16:35:30","guid":{"rendered":"https:\/\/blog.ineuro.net\/?p=515"},"modified":"2023-09-09T00:36:28","modified_gmt":"2023-09-08T16:36:28","slug":"%e6%96%87%e7%8c%ae%e5%ad%a6%e4%b9%a02%e5%88%9b%e4%bc%a4%e6%80%a7%e8%84%91%e6%8d%9f%e4%bc%a4%e5%af%bc%e8%87%b4%e7%94%b1%e5%b0%8f%e8%83%b6%e8%b4%a8%e7%bb%86%e8%83%9e%e4%bb%8b%e5%af%bc%e7%9a%84%e6%85%a2","status":"publish","type":"post","link":"https:\/\/blog.ineuro.net\/index.php\/2023\/09\/09\/515\/","title":{"rendered":"\u6587\u732e\u5b66\u4e602:\u521b\u4f24\u6027\u8111\u635f\u4f24\u5bfc\u81f4\u7531\u5c0f\u80f6\u8d28\u7ec6\u80de\u4ecb\u5bfc\u7684\u6162\u6027\u76ae\u8d28\u708e\u75c7\u548c\u795e\u7ecf\u5143\u529f\u80fd\u969c\u788d"},"content":{"rendered":"\n<p>\u6587\u732e\u5b66\u4e60<\/p>\n\n\n\n<p>Title\uff1aTraumatic Brain Injury Causes Chronic Cortical Inflammation and Neuronal Dysfunction Mediated by Microglia<\/p>\n\n\n\n<p>DOI: https:\/\/doi.org\/10.1523\/JNEUROSCI.2469-20.2020<\/p>\n\n\n\n<p>\u9898\u76ee\uff1a\u521b\u4f24\u6027\u8111\u635f\u4f24\u5bfc\u81f4\u7531\u5c0f\u80f6\u8d28\u7ec6\u80de\u4ecb\u5bfc\u7684\u6162\u6027\u76ae\u8d28\u708e\u75c7\u548c\u795e\u7ecf\u5143\u529f\u80fd\u969c\u788d<\/p>\n\n\n\n<p>\u521b\u4f24\u6027\u8111\u635f\u4f24\uff08TBI\uff09\u53ef\u80fd\u5bfc\u81f4\u663e\u8457\u7684\u795e\u7ecf\u7cbe\u795e\u95ee\u9898\u548c\u795e\u7ecf\u9000\u884c\u6027\u75c5\u7406\uff0c\u8fd9\u4e9b\u95ee\u9898\u5728\u53d7\u4f24\u540e\u591a\u5e74\u4ecd\u7136\u5b58\u5728\u548c\u53d1\u5c55\u3002\u4e0e\u6b64\u540c\u65f6\uff0c\u795e\u7ecf\u708e\u75c7\u8fc7\u7a0b\u4e5f\u5728\u8fd9\u6bb5\u65f6\u95f4\u5185\u6f14\u53d8\u3002\u56e0\u6b64\uff0c\u7814\u7a76\u4eba\u5458\u7684\u76ee\u6807\u662f\u786e\u5b9a\u5c0f\u80f6\u8d28\u7ec6\u80de\u5728\u521b\u4f24\u540e\u6025\u6027\uff081\u5929\uff09\u3001\u4e9a\u6025\u6027\uff087\u5929\uff09\u548c\u6162\u6027\uff0830\u5929\uff09\u65f6\u671f\u5bf9\u795e\u7ecf\u75c5\u7406\u7684\u8d21\u732e\u3002\u5728\u7ed9\u96c4\u6027\u5c0f\u9f20\u8fdb\u884c\u4e2d\u7ebf\u6db2\u4f53\u51b2\u51fb\u4f24\uff08FPI\uff09\u4e4b\u524d\uff0c\u4f7f\u7528CSF1R\u62ee\u6297\u5242PLX5622\u53bb\u9664\u4e86\u5c0f\u80f6\u8d28\u7ec6\u80de\uff0c\u5e76\u4f7f\u7528\u795e\u7ecf\u75c5\u7406mRNA\u9762\u677f\u8bc4\u4f30\u4e86\u76ae\u5c42\u795e\u7ecf\u75c5\u7406\/\u708e\u75c7\u3002\u53d7\u4f24\u540e\u6025\u6027\u65f6\u671f\uff081\u5929\uff09\u4e0e\u708e\u75c7\u548c\u795e\u7ecf\u75c5\u7406\u6709\u5173\u7684\u57fa\u56e0\u8868\u8fbe\u5927\u5e45\u589e\u52a0\uff0c\u8fd9\u4e9b\u8868\u8fbe\u7684\u5927\u90e8\u5206\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u65e0\u5173\u3002\u7136\u800c\uff0c\u57287\u5929\u548c30\u5929\u65f6\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u9006\u8f6c\u4e86\u4e0eTBI\u76f8\u5173\u7684\u4e0e\u708e\u75c7\u3001\u5e72\u6270\u7d20\u4fe1\u53f7\u548c\u795e\u7ecf\u75c5\u7406\u6709\u5173\u7684\u57fa\u56e0\u8868\u8fbe\u3002\u5728\u4e9a\u6025\u6027\u548c\u6162\u6027\u7684\u7ec8\u70b9\uff0c\u5927\u91cf\u88ab\u6291\u5236\u7684\u57fa\u56e0\u5f52\u56e0\u4e8e\u795e\u7ecf\u5143\u3002\u4e3a\u4e86\u7406\u89e3\u5c0f\u80f6\u8d28\u7ec6\u80de\u3001\u795e\u7ecf\u5143\u548c\u5176\u4ed6\u80f6\u8d28\u7ec6\u80de\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u7814\u7a76\u4eba\u5458\u57287\u5929\u5b8c\u6210\u4e86\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff0c\u8fd9\u662f\u4ece\u6025\u6027\u5230\u6162\u6027\u53d1\u75c5\u6f14\u53d8\u7684\u5173\u952e\u65f6\u671f\u3002\u76ae\u5c42\u5c0f\u80f6\u8d28\u7ec6\u80de\u8868\u73b0\u51fa\u4e0eTBI\u76f8\u5173\u7684\u72ec\u7279\u805a\u7c7b\uff0c\u4e0etype-1\u5e72\u6270\u7d20\u548c\u4e0e\u795e\u7ecf\u9000\u884c\u6027\/\u635f\u4f24\u76f8\u5173\u7684\u57fa\u56e0\u589e\u52a0\u3002\u5728\u76ae\u5c42\u795e\u7ecf\u5143\u4e2d\uff0c\u4e0e\u591a\u5df4\u80fa\u4fe1\u53f7\u3001\u957f\u65f6\u7a0b\u589e\u5f3a\u3001\u9499\u4fe1\u53f7\u548c\u7a81\u89e6\u751f\u6210\u6709\u5173\u7684\u57fa\u56e0\u88ab\u6291\u5236\u3002\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u9006\u8f6c\u4e86\u795e\u7ecf\u5143\u7684\u5927\u90e8\u5206\u8fd9\u4e9b\u6539\u53d8\u3002\u6b64\u5916\uff0c7\u5929\u65f6\u76ae\u5c42\u6811\u7a81\u590d\u6742\u5ea6\u51cf\u5c11\uff0c30\u5929\u65f6\u795e\u7ecf\u5143\u8fde\u63a5\u6027\u51cf\u5c11\uff0c30\u5929\u65f6\u51fa\u73b0\u8ba4\u77e5\u969c\u788d\u3002\u8fd9\u4e9b\u4e0eTBI\u76f8\u5173\u7684\u529f\u80fd\u548c\u884c\u4e3a\u969c\u788d\u90fd\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u800c\u5f97\u5230\u9884\u9632\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u4e9b\u7814\u7a76\u8868\u660e\uff0c\u5728TBI\u540e\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u4fc3\u8fdb\u6301\u7eed\u7684\u795e\u7ecf\u75c5\u7406\u548c\u795e\u7ecf\u5143\u7a33\u6001\u7684\u957f\u671f\u529f\u80fd\u969c\u788d\u3002<\/p>\n\n\n\n<h1 class=\"wp-block-heading\">\u4e00\u3001\u7814\u7a76\u80cc\u666f<\/h1>\n\n\n\n<p>\u521b\u4f24\u6027\u8111\u635f\u4f24\uff08TBI\uff09\u662f\u7f8e\u56fd\u795e\u7ecf\u548c\u795e\u7ecf\u7cbe\u795e\u969c\u788d\u7684\u4e3b\u8981\u539f\u56e0\u3002\u4f8b\u5982\uff0cTBI\u60a3\u8005\u60a3\u6709\u6291\u90c1\u75c7\u7684\u51e0\u7387\u662f\u666e\u901a\u4eba\u7fa4\u76845-10\u500d\uff0c\u800c30%\u7684TBI\u60a3\u8005\u663e\u793a\u51fa\u53ef\u68c0\u6d4b\u7684\u8ba4\u77e5\u4e0b\u964d\u3002\u6700\u8fd1\uff0c\u4e3a\u6d3b\u4f53\u68c0\u6d4bTBI\u540e\u7684\u708e\u75c7\uff0c\u5f00\u53d1\u4e86\u53ef\u4ee5\u4e0e\u6d3b\u5316\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\u7ed3\u5408\u7684PET\u53ef\u68c0\u6d4b\u914d\u4f53\u3002\u957f\u8fbe17\u5e74\u540e\u7684TBI\u60a3\u8005\u4e2d\u68c0\u6d4b\u5230\u4e86\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e0a\u7684\u82ef\u4e8c\u6c2e\u5e73\u53d7\u4f53\u7ed3\u5408\u589e\u52a0\u7684PK11195\uff0c\u5e76\u4e0e\u8ba4\u77e5\u5904\u7406\u901f\u5ea6\u53d7\u635f\u76f8\u5173\u3002\u5c3d\u7ba1\u76ee\u524d\u6ca1\u6709\u9488\u5bf9TBI\u76f8\u5173\u795e\u7ecf\u7cbe\u795e\u540e\u9057\u75c7\u7684\u836f\u7269\u7597\u6cd5\uff0c\u4f46\u5c0f\u80f6\u8d28\u7ec6\u80de\u5728\u53d7\u4f24\u540e\u51e0\u4e2a\u6708\u5230\u51e0\u5e74\u7684\u7a33\u6001\u5f02\u5e38\u6539\u53d8\u53ef\u80fd\u4ee3\u8868\u4e86\u6cbb\u7597\u5e72\u9884\u7684\u673a\u4f1a\u3002<\/p>\n\n\n\n<p>\u5c0f\u9f20\u7684TBI\u6a21\u578b\u4e2d\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u7ed3\u6784\u548c\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u708e\u75c7\u7684\u6162\u6027\u7d0a\u4e71\u5df2\u5f97\u5230\u6a21\u62df\u3002\u5c0f\u9f20\u91cd\u590d\u7684\u95ed\u5408\u5934\u90e8\u635f\u4f24\u5bfc\u81f4\u4e86\u5c0f\u80f6\u8d28\u7ec6\u80de\uff08Iba1\uff09\u7684\u6807\u8bb0\u589e\u52a0\uff0c\u4e0e\u4f24\u540e12-18\u4e2a\u6708\u7684\u6d77\u9a6c\u4f9d\u8d56\u6027\u5b66\u4e60\u969c\u788d\u76f8\u4e00\u81f4\u3002\u4e00\u7cfb\u5217\u7684\u7814\u7a76\u8bc1\u5b9e\u4e86\u53d7\u4f24\u540e\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u708e\u75c7\u6d3b\u5316\u3002<\/p>\n\n\n\n<p>\u5c0f\u80f6\u8d28\u7ec6\u80de\u53c2\u4e0e\u591a\u79cd\u7a33\u6001\u8fc7\u7a0b\uff0c\u5305\u62ec\u7a81\u89e6\u4fee\u526a\u3001\u514d\u75ab\u76d1\u6d4b\u548c\u788e\u7247\u6e05\u9664\uff1b\u56e0\u6b64\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u7a33\u6001\u7684\u957f\u671f\u7834\u574f\u53ef\u80fd\u662f\u53d7\u4f24\u540e\u7684\u795e\u7ecf\u7cbe\u795e\u5e76\u53d1\u75c7\u548c\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u7684\u6839\u6e90\u3002<\/p>\n\n\n\n<p>\u5927\u91cf\u7ec4\u7ec7\u5b66\u8bc1\u636e\u8868\u660eTBI\u540e\u795e\u7ecf\u5143\u548c\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e4b\u95f4\u5b58\u5728\u52a8\u6001\u7ed3\u6784\u5173\u8054\u3002\u4f46\u662f\uff0cTBI\u540e\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e0e\u795e\u7ecf\u5143\u7684\u7ed3\u6784\u4e92\u52a8\u7684\u529f\u80fd\u610f\u4e49\u5c1a\u4e0d\u6e05\u695a\u3002<\/p>\n\n\n\n<p>\u8fd9\u9879\u7814\u7a76\u7684\u4e3b\u8981\u76ee\u6807\u662f\u786e\u5b9a\u5c0f\u80f6\u8d28\u7ec6\u80de\u662f\u5426\u662fTBI\u540e\u795e\u7ecf\u75c5\u7406\u7684\u9a71\u52a8\u56e0\u7d20\u3002\u7814\u7a76\u4eba\u5458\u4e4b\u524d\u7684\u7814\u7a76\u5df2\u7ecf\u663e\u793a\uff0c\u5728\u53d7\u4f24\u524d\u548c\u53d7\u4f24\u540e7\u5929\u901a\u8fc7CSF1R\u62ee\u6297\u6d88\u9664\u5c0f\u80f6\u8d28\u7ec6\u80de\u53ef\u4ee5\u9884\u9632\u4e2d\u7ebfFPI\uff08mFPI\uff09\u540e\u591a\u79cd\u514d\u75ab\u548c\u708e\u75c7\u57fa\u56e0\u7684\u8868\u8fbe\u3002\u5f53\u524d\u7814\u7a76\u7684\u76ee\u6807\u662f\u53cc\u91cd\u7684\uff1a\u9996\u5148\uff0c\u4e86\u89e3\u4ece\u6025\u6027\uff081\u5929\uff09\u548c\u4e9a\u6025\u6027\uff087\u5929\uff09\u5230\u6162\u6027\uff0830\u5929\uff09\u635f\u4f24\u9636\u6bb5\u5c0f\u80f6\u8d28\u7ec6\u80de\u5728\u795e\u7ecf\u75c5\u7406\u57fa\u56e0\u8868\u8fbe\u8fdb\u5c55\u4e2d\u7684\u4f5c\u7528\uff1b\u5176\u6b21\uff0c\u4e86\u89e3\u5c0f\u80f6\u8d28\u7ec6\u80de\u662f\u5426\u4ecb\u5bfc\u795e\u7ecf\u5143\u5bf9\u635f\u4f24\u7684\u8f6c\u5f55\u3001\u7ed3\u6784\u548c\u751f\u7406\u53cd\u5e94\u3002\u7814\u7a76\u4eba\u5458\u7684\u6570\u636e\u7ed3\u679c\u8868\u660e\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u662f\u6162\u6027\u708e\u75c7\u7684\u5173\u952e\u4ecb\u8d28\uff0c\u5e76\u5728\u8f6c\u5f55\u3001\u7ed3\u6784\u3001\u751f\u7406\u548c\u529f\u80fd\u5c42\u9762\u6291\u5236\u795e\u7ecf\u5143\u7684\u7a33\u6001\u3002<\/p>\n\n\n\n<h1 class=\"wp-block-heading\">\u4e8c\u3001\u7814\u7a76\u65b9\u6cd5<\/h1>\n\n\n\n<h2 class=\"wp-block-heading\">1.\u5b9e\u9a8c\u52a8\u7269<\/h2>\n\n\n\n<p>8-10\u5468\u5927\u7684C57BL\/6\u96c4\u6027\u5c0f\u9f20\u4eceCharles River Breeding Laboratories\u8d2d\u5f97\u3002\u4e3a\u4e86\u8fdb\u884c\u795e\u7ecf\u8ffd\u8e2a\u548c\u6811\u7a81\u68d8\u5206\u6790\uff0c\u4eceThe Jackson Laboratory\u8d2d\u5f97Thy1-YFP-H\u5c0f\u9f20\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">2.PLX5622\u7ed9\u836f<\/h2>\n\n\n\n<p>PLX5622\u7531Plexxikon Inc.\u63d0\u4f9b\uff0c\u5e76\u7531Research Diets\u5728AIN-76A\u556e\u9f7f\u52a8\u7269\u9972\u6599\u4e2d\u914d\u5236\u62101200mg\/kg\u7684\u6d53\u5ea6\u3002\u6807\u51c6\u7684AIN-76A\u996e\u98df\u4f5c\u4e3a\u8f7d\u4f53\u63a7\u5236\u3002\u4e3a\u4e86\u5728TBI\u524d\u6d88\u8017\u5c0f\u80f6\u8d28\u7ec6\u80de\uff0c\u5c0f\u9f20\u8fde\u7eed14\u5929\u968f\u610f\u8fdb\u98dfPLX5622\u6216\u8f7d\u4f53\u996e\u98df\uff0c\u5e76\u5728\u6574\u4e2a\u7814\u7a76\u671f\u95f4\u7ee7\u7eed\u8fd9\u79cd\u996e\u98df\u3002\u5b9e\u9a8c\u5ba4\u9a8c\u8bc1\u4e86\u8fd9\u4e2a\u5242\u91cf\u548c\u65f6\u95f4\u53ef\u4ee5\u5728C57BL\/6\u5c0f\u9f20\u4e2d\u6d88\u8017\u5927\u7ea696%\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">3.\u76ae\u5c42\u5fae\u5207\u5206\u6790NanoString<\/h2>\n\n\n\n<p>\u76ae\u8d28\u5fae\u89e3\u5256\u7528\u4e8eNanoString \u4ece\u5305\u542b\u80a5\u5927\u548c\u68d2\u72b6\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u76ae\u8d28\u5fae\u89e3\u5256\u533a\u57df\u63d0\u53d6RNA\uff08Witcher et al., 2018\uff09\u3002\u5728\u5904\u6b7b\u65f6\uff0c\u8fc5\u901f\u53d6\u51fa\u5927\u8111\u5e76\u5728\u5e72\u51b0\u51b7\u5374\u7684\u5f02\u620a\u70f7\u4e2d\u5feb\u901f\u51b7\u51bb\u3002\u65b0\u9c9c\u51b7\u51bb\u7684\u7ec4\u7ec7\u4ea4\u66ff\u5207\u621030\u5fae\u7c73\u548c200\u5fae\u7c73\u539a\uff0c\u7136\u540e\u8d34\u5728\u5e26\u7535\u5e7b\u706f\u7247\u4e0a\u3002\u8584\u7247\u6216\u5bfc\u5411\u7247\u4f7f\u7528CD11b\uff081:300, \u751f\u7269\u7d20\u5316\uff09\u6807\u8bb0\uff0c\u5e76\u4f7f\u7528ABC\u6eb6\u6db2\uff08Vector Laboratories\uff09\u548c\u4e8c\u6c28\u57fa\u82ef\u80fa\uff08DAB\uff09\u8fc7\u6c27\u5316\u7269\u5e95\u7269\u8bd5\u5242\u76d2\uff08Vector Laboratories\uff09\u8fdb\u884c\u53ef\u89c6\u5316\u3002\u4f7f\u7528\u6807\u8bb0\u7684\u5bfc\u5411\u7247\u5728\u51b7\u51bb\u7684\u539a\u5207\u7247\u4e2d\u8bc6\u522b\u611f\u5174\u8da3\u7684\u533a\u57df\uff0c\u7136\u540e\u8fdb\u884c\u5fae\u5207\u5272\uff0c\u7acb\u5373\u88c2\u89e3\uff0c\u5e76\u63d0\u53d6RNA\uff08PicoPure RNA\u63d0\u53d6\u8bd5\u5242\u76d2\uff0cThermo Fisher Scientific\uff09\u3002\u4f7f\u7528Agilent BioAnalyzer\u786e\u8ba4RNA\u7684\u5b8c\u6574\u6027\uff0c\u5e76\u4f7f\u7528housekeeping\u57fa\u56e0\uff08Gapdh, Actb\uff09\u7684\u8868\u8fbe\u786e\u5b9a\u9884\u653e\u5927\u5468\u671f\uff1b\u6bcf\u4e2a\u5b9e\u9a8c\u7ec45-9\u53ea\u5c0f\u9f20\u3002<\/p>\n\n\n\n<p>\u9644<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u7f8e\u56fdNanoString \u662f\u7ee7\u751f\u7269\u82af\u7247\u6280\u672f\u548c\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\uff08NGS)\u540e\u5728\u57fa\u56e0\u8868\u8fbe\u8c31\u5206\u6790\u4e0a\u5c55\u793a\u51fa\u5f3a\u5927\u5e94\u7528\u524d\u666f\u7684\u65b0\u6280\u672f\u516c\u53f8\u3002\u5176\u6570\u5b57\u57fa\u56e0\u8868\u8fbe\u8c31\u6280\u672fNanoString nCounter\uff1f\u5206\u6790\u7cfb\u7edf\uff0c\u5229\u7528\u4e13\u5229\u5206\u5b50\u6761\u5f62\u7801\u53ca\u5355\u5206\u5b50\u6210\u50cf\u6280\u672f\uff0c\u8fdb\u884c\u9ad8\u901a\u91cf\u591a\u91cd\u57fa\u56e0\u8868\u8fbe\u7814\u7a76\uff0c\u7cfb\u7edf\u76f4\u63a5\u5bf9\u591a\u79cd\u6837\u672c\u4e2d\u7684\u8f6c\u5f55\u672c\u8fdb\u884c\u6570\u5b57\u5316\u68c0\u6d4b\u3002\u4e0d\u9700\u53cd\u8f6c\u5f55\u53ca\u540e\u7eedPCR\u6269\u589e\uff0c\u4ea7\u51fa\u6570\u636e\u7cbe\u51c6\u7a33\u5b9a\uff0c\u53ef\u518d\u73b0\u6027\u9ad8\u3002\u5176GeoMx DSP\u6570\u5b57\u5f0f\u7a7a\u95f4\u591a\u9776\u6807\u5206\u6790\u7cfb\u7edf\uff0c\u53ef\u5b9e\u73b0\u7ec4\u7ec7\u5207\u7247\u6c34\u5e73\u591a\u7ef4\u5ea6\uff08RNA\u548c\u86cb\u767d\u6c34\u5e73\uff09\u3001\u591a\u91cd\uff08Multi-plex\uff09\u4f4d\u7f6e\u4fe1\u606f\u6316\u6398<\/p>\n<\/blockquote>\n\n\n\n<h2 class=\"wp-block-heading\">4.NanoString\u57fa\u56e0\u8868\u8fbe\u5206\u6790<\/h2>\n\n\n\n<p>\u4f7f\u7528NanoString neuropathology\u9762\u677f\u5bf9\u57fa\u56e0\u8868\u8fbe\u8fdb\u884c\u5b9a\u91cf\uff0c\u589e\u52a0\u4e8630\u4e2a\u81ea\u5b9a\u4e49\u57fa\u56e0\uff08NanoString Technologies\uff09\u3002\u9996\u5148\u6839\u636e\u5236\u9020\u5546\u7684\u534f\u8bae\u5bf9\u6b63\u8d1f\u63a7\u5236\u8fdb\u884c\u6280\u672f\u89c4\u8303\u5316\u3002\u57fa\u4e8e\u4e0e\u603b\u8ba1\u6570\u7684\u5f3a\u76f8\u5173\u6027\u9a8c\u8bc1\u4e86housekeeping\u57fa\u56e0\uff0c\u5e76\u4f7f\u7528\u4e03\u4e2a\u57fa\u56e0\u5bf9\u6570\u636e\u8fdb\u884c\u89c4\u8303\u5316\uff08Aars, Asb7, Ccdc127, Cnot10, Csnk2a2, Lars, Mto1\uff09\u3002\u4f7f\u7528DESeq2\u5728R\u4e2d\u8fdb\u884c\u6570\u636e\u89c4\u8303\u5316\u548c\u5dee\u5f02\u8868\u8fbe\u6d4b\u8bd5\uff08Love et al., 2014\uff09\u3002\u4f7f\u7528ingenuity pathway analysis\uff08IPA\uff1bQIAGEN\uff09\u8fdb\u884c\u901a\u8def\u5206\u6790\u3002\u539f\u59cb\u7684RCC\u6587\u4ef6\u548c\u89c4\u8303\u5316\u7684\u8ba1\u6570\u77e9\u9635\u5728NCBI\u7684Gene Expression Omnibus\uff08GEO\uff1bGSE160651\uff09\u4e2d\u53ef\u7528\uff1b\u5728\u6269\u5c55\u6570\u636e\u56fe1-2\u4e2d\u63d0\u4f9b\u4e86\u539f\u59cb\u548c\u89c4\u8303\u5316\u7684\u8ba1\u6570\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">5.\u76ae\u5c42\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u89e3\u79bb\uff1a<\/h2>\n\n\n\n<p>\u5c0f\u9f20\u7528\u51b0\u51b7PBS\u704c\u6ce8\uff0c\u5927\u8111\u8fc5\u901f\u53d6\u51fa\uff0c\u76ae\u5c42\u8fdb\u884c\u663e\u5fae\u5207\u5272\u3002\u76ae\u5c42\u7528\u6210\u5e74\u5c0f\u9f20\u8111\u89e3\u79bb\u8bd5\u5242\u76d2\uff08\u9002\u7528\u4e8e\u5c0f\u9f20\u548c\u5927\u9f20\uff09\u6309\u7167\u5236\u9020\u5546\u7684\u8bf4\u660e\u8fdb\u884c\u89e3\u79bb\uff0c\u4f46\u6709\u8f7b\u5fae\u7684\u4fee\u6539 (Miltenyi Biotec)\u3002\u4eba\u9020CSF (aCSF\uff0c\u5982\u4e0b\u6240\u8ff0) \u7528\u4e8e\u5236\u5907\u9176\u6eb6\u6db2\u3002\u5207\u4e0b\u7684\u76ae\u5c42\u7acb\u5373\u653e\u5165\u9884\u70ed(37\u00b0C)\u7684\u9176P\u6eb6\u6db2\u4e2d\uff0c\u52a0\u5165\u9176A\u6eb6\u6db2\uff0c\u5e76\u5728C\u7ba1\u4e2d\u572837\u00b0C\u4e0b\u4f7f\u7528gentleMACS\u7ec6\u80de\u89e3\u79bb\u5668 (Miltenyi Biotec) \u8fdb\u884c\u89e3\u79bb\u3002\u5bc6\u5ea6\u79bb\u5fc3\u6cd5\u7528\u4e8e\u53bb\u9664\u9ad3\u9798\u788e\u7247\uff0c\u5269\u4f59\u7684\u7ea2\u7ec6\u80de\u88ab\u7834\u88c2\uff0c\u4f7f\u7528\u8840\u7ec6\u80de\u8ba1\u6570\u5668\u786e\u5b9a\u7ec6\u80de\u7684\u6d3b\u529b\u548c\u6d53\u5ea6\u3002\u6bcf\u7ec4\u4e2d\u7684\u4e09\u53ea\u5c0f\u9f20\u7684\u76ae\u5c42\uff08Veh-Con\uff0cVehTBI\uff0cPLX5622-Con\uff0cPLX5622-TBI\uff09\u88ab\u6c47\u96c6\u5230\u6bcf\u4e2a\u6837\u672c\u4e2d\u3002\u8be5\u5b9e\u9a8c\u91cd\u590d\u4e24\u6b21\uff0c\u6bcf\u4e2a\u5b9e\u9a8c\u7ec4\u4f7f\u7528\u4e86\u516d\u53ea\u5c0f\u9f20\u3002\u6bcf\u4e2a\u76ae\u5c42\u5300\u6d46\u6062\u590d\u7ea63000-7000\u4e2a\u7ec6\u80de\u3002<\/p>\n\n\n\n<p>10x Genomics\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff1a \u5355\u7ec6\u80de\u76ae\u5c42\u60ac\u6d6e\u6db2\u52a0\u8f7d\u523010x\u82af\u7247\u4e0a\uff0c\u5e76\u5728\u67d3\u8272\u4f53\u63a7\u5236\u5668\u4e0a\u8fd0\u884c\u4ee5\u4ea7\u751f\u51dd\u80f6\u73e0\u4e73\u6db2(10x Genomics)\u3002UMI\u6761\u5f62\u7801\u3001cDNA\u6269\u589e\u548c\u6587\u5e93\u6784\u5efa\u7684\u534f\u8bae\u9075\u5faa10x Genomics\u5355\u7ec6\u80de3&#8242; v2\u8bd5\u5242\u76d2\u7684\u534f\u8bae\u3002\u5229\u7528Agilent\u9ad8\u7075\u654f\u5ea6DNA BioAnalyzer\u82af\u7247\u786e\u5b9a\u6587\u5e93\u8d28\u91cf\uff0c\u5e76\u4f7f\u7528Illumina HiSeq\u8fdb\u884c\u6d4b\u5e8f\u3002\u4f7f\u7528CellRanger (10x Genomics) \u5c06\u6d4b\u5e8f\u8bfb\u53d6\u5bf9\u9f50\u5230mm10\u5c0f\u9f20\u57fa\u56e0\u7ec4\uff0c\u5e76\u751f\u6210\u8ba1\u6570\u77e9\u9635\u3002NCBI\u7684\u57fa\u56e0\u8868\u8fbe\u7efc\u89c8(GEO; GSE160763)\u63d0\u4f9b\u4e86\u539f\u59cbFASTQ\u6587\u4ef6\u548c\u8ba1\u6570\u77e9\u9635\u3002\u4f7f\u7528Seurat\u5728R\u4e2d\u8fc7\u6ee4\u4f4e\u8d28\u91cf\u7ec6\u80de(\u53cc\u5cf0\u3001\u4f4e\u8ba1\u6570\u3001\u9ad8\u8ba1\u6570) (Butler\u7b49\u4eba\uff0c2018\u5e74)\u3002\u6839\u636e\u663e\u8457\u589e\u52a0\u7684\u57fa\u56e0\u8868\u8fbe\u5c06\u7ec6\u80de\u7c7b\u578b\u5206\u914d\u7ed9\u6bcf\u4e2a\u7c07\u3002\u4ece\u5b8c\u6574\u6570\u636e\u96c6\u4e2d\u63d0\u53d6\u4e86Tmem1191 microglia\u3001Gja11\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u3001Plp11\u9ad3\u9798\u7ec6\u80de\u548cMeg31\u795e\u7ecf\u5143\uff0c\u5e76\u8fdb\u4e00\u6b65\u5206\u6790\u3002\u4f7f\u7528Seurat (p-adj &lt; 0.05) \u786e\u5b9a\u4e86\u7c07\u7279\u5f02\u6027\u8868\u8fbe\u548c\u5b9e\u9a8c\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u8868\u8fbe\u3002\u6269\u5c55\u6570\u636e\u56fe3-2\u5305\u542b\u7528\u4e8e\u786e\u5b9a\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684Veh-Con\u548cVeh-TBI\u7ec4\u4e4b\u95f4\u7684\u7c07\u7279\u5f02\u6027\u6807\u8bb0\u7269\u548c\u5dee\u5f02\u8868\u8fbe\u7684\u5206\u6790\u7684\u539f\u59cb\u8f93\u51fa<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">6.\u5927\u8111\u76ae\u5c42\u89e3\u79bb\u7528\u4e8e\u5355\u7ec6\u80de\u6d4b\u5e8f<\/h2>\n\n\n\n<p>\u5c0f\u9f20\u7528\u51b0\u51b7PBS\u704c\u6d41\uff0c\u5927\u8111\u8fc5\u901f\u53d6\u51fa\uff0c\u5e76\u5bf9\u76ae\u8d28\u8fdb\u884c\u5fae\u5207\u5272\u3002\u4f7f\u7528Miltenyi Biotec\u516c\u53f8\u7684\u6210\u5e74\u5c0f\u9f20\u8111\u89e3\u79bb\u5957\u4ef6\u5bf9\u5c0f\u9f20\u548c\u5927\u9f20\u7684\u76ae\u8d28\u8fdb\u884c\u89e3\u79bb\uff0c\u6309\u7167\u751f\u4ea7\u5546\u7684\u8bf4\u660e\u8fdb\u884c\u4e86\u5c11\u91cf\u4fee\u6539\u3002\u4eba\u5de5\u8111\u810a\u6db2\uff08aCSF\uff0c\u5982\u4e0b\u6240\u8ff0\uff09\u7528\u4e8e\u5236\u5907\u9176\u6eb6\u6db2\u3002\u5207\u4e0b\u7684\u76ae\u8d28\u7acb\u5373\u653e\u5165\u9884\u70ed\uff0837\u00b0C\uff09\u7684\u9176P\u6eb6\u6db2\u4e2d\uff0c\u6dfb\u52a0\u9176A\u6eb6\u6db2\uff0c\u7136\u540e\u572837\u00b0C\u4e0b\u4f7f\u7528gentleMACS\u89e3\u79bb\u5668\uff08Miltenyi Biotec\uff09\u5728C\u7ba1\u4e2d\u5bf9\u7ec4\u7ec7\u8fdb\u884c\u89e3\u79bb\u3002\u7528\u5bc6\u5ea6\u79bb\u5fc3\u6cd5\u53bb\u9664\u9ad3\u9798\u788e\u7247\uff0c\u6eb6\u89e3\u5269\u4f59\u7684\u7ea2\u7ec6\u80de\uff0c\u4f7f\u7528\u8840\u7403\u8ba1\u6570\u4eea\u6d4b\u5b9a\u7ec6\u80de\u7684\u6d3b\u6027\u548c\u6d53\u5ea6\u3002\u4ece\u6bcf\u4e2a\u5b9e\u9a8c\u7ec4\uff08Veh-Con\uff0cVehTBI\uff0cPLX5622-Con\uff0cPLX5622-TBI\uff09\u7684\u4e09\u53ea\u5c0f\u9f20\u7684\u76ae\u5c42\u4e2d\u6c47\u96c6\u6837\u672c\u3002\u8fd9\u4e2a\u5b9e\u9a8c\u8fdb\u884c\u4e86\u4e24\u6b21\u590d\u5236\uff0c\u56e0\u6b64\u6bcf\u4e2a\u5b9e\u9a8c\u7ec4\u4f7f\u7528\u4e86\u516d\u53ea\u5c0f\u9f20\u3002\u7814\u7a76\u4eba\u5458\u4ece\u6bcf\u4e2a\u76ae\u8d28\u5300\u6d46\u4e2d\u56de\u6536\u4e86\u5927\u7ea63000-7000\u4e2a\u7ec6\u80de\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">7.10x Genomics\u5355\u7ec6\u80deRNA\u6d4b\u5e8f<\/h2>\n\n\n\n<p>\u5355\u7ec6\u80de\u76ae\u8d28\u60ac\u6d6e\u6db2\u88c5\u8f7d\u523010x\u82af\u7247\u4e0a\uff0c\u5728Chromium Controller\u4e0a\u8fd0\u884c\uff0c\u751f\u6210\u51dd\u80f6\u73e0\u4e73\u72b6\u6db2\uff0810x Genomics\uff09\u3002\u6309\u716710x Genomics Single Cell 39 v2\u8bd5\u5242\u76d2\u534f\u8bae\u8fdb\u884cUMI\u6761\u5f62\u7801\u3001cDNA\u6269\u589e\u548c\u6587\u5e93\u6784\u5efa\u3002\u4f7f\u7528Agilent High Sensitivity DNA BioAnalyzer\u82af\u7247\u786e\u5b9a\u6587\u5e93\u8d28\u91cf\uff0c\u5e76\u4f7f\u7528Illumina HiSeq\u8fdb\u884c\u6d4b\u5e8f\u3002\u6d4b\u5e8f\u8bfb\u6570\u6bd4\u5bf9\u5230mm10\u5c0f\u9f20\u57fa\u56e0\u7ec4\uff0c\u4f7f\u7528CellRanger\uff0810x Genomics\uff09\u751f\u6210\u8ba1\u6570\u77e9\u9635\u3002\u539f\u59cbFASTQ\u6587\u4ef6\u548c\u8ba1\u6570\u77e9\u9635\u5728NCBI GEO\uff08GSE160763\uff09\u4e2d\u53ef\u7528\u3002<strong>\u4f7f\u7528Seurat in R\uff08Butler\u7b49\u4eba\uff0c2018\uff09\u8fc7\u6ee4\u4f4e\u8d28\u91cf\u7684\u7ec6\u80de\uff08\u53cc\u4f53\u7ec6\u80de\u3001\u4f4e\u8ba1\u6570\u3001\u9ad8\u8ba1\u6570\uff09\u3002\u57fa\u4e8e\u663e\u8457\u589e\u52a0\u7684\u57fa\u56e0\u8868\u8fbe\u4e3a\u6bcf\u4e2a\u7c07\u5206\u914d\u7ec6\u80de\u7c7b\u578b\u3002\u4ece\u5b8c\u6574\u7684\u6570\u636e\u96c6\u4e2d\u63d0\u53d6\u4e86Tmem1191\u5c0f\u80f6\u8d28\u7ec6\u80de\u3001Gja11\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u3001Plp11\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u548cMeg31\u795e\u7ecf\u5143\uff0c\u5e76\u8fdb\u884c\u4e86\u8fdb\u4e00\u6b65\u7684\u5206\u6790\u3002\u4f7f\u7528Seurat\u786e\u5b9a\u7c07\u7279\u5f02\u6027\u8868\u8fbe\u548c\u5b9e\u9a8c\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u8868\u8fbe<\/strong>\uff08p-adj &lt; 0.05\uff09\u3002\u6269\u5c55\u6570\u636e\u56fe3-2\u5305\u542b\u4e86\u786e\u5b9a\u7c07\u7279\u5f02\u6027\u6807\u8bb0\u7269\u548cVeh-Con\u4e0eVeh-TBI\u7ec4\u4e4b\u95f4\u7684\u6bcf\u4e2a\u7ec6\u80de\u7c7b\u578b\u7684\u5dee\u5f02\u8868\u8fbe\u7684\u5206\u6790\u7ed3\u679c\u7684\u539f\u59cb\u8f93\u51fa\u3002<\/p>\n\n\n\n<p>\u4e3a\u786e\u5b9aPLX5622\u5bf9TBI\u7684\u9006\u8f6c\u4f5c\u7528\uff0c\u786e\u5b9a\u53d7TBI\u5f71\u54cd\u7684\u57fa\u56e0\uff1a\uff081\uff09\u5982\u679c\u8868\u8fbe\u5728PLX-TBI\u7ec4\u4e2d\u8fd4\u56de\u5230\u57fa\u7ebf\uff08Veh-Con\uff09\u6c34\u5e73\uff0c\u4e14Veh-TBI\u4e0ePLX-TBI\u4e4b\u95f4\u663e\u8457\u4e0d\u540c\uff0c\u5219\u88ab\u8ba4\u4e3a\u662f\u9006\u8f6c\u7684\uff1b\uff082\uff09\u5982\u679c\u8868\u8fbe\u8fd4\u56de\u5230\u57fa\u7ebf\uff08Veh-Con\uff09\uff0c\u4f46Veh-TBI\u548cPLX-TBI\u4e4b\u95f4\u4e0d\u663e\u8457\uff0c\u5219\u88ab\u8ba4\u4e3a\u662f\u90e8\u5206\u9006\u8f6c\u7684\uff1b\uff083\uff09\u5982\u679cPLX-TBI\u4e0e\u57fa\u7ebf\u663e\u8457\u6539\u53d8\u7684\u65b9\u5411\u76f8\u540c\uff0c\u5982Veh-TBI\uff0c\u5219\u88ab\u8ba4\u4e3a\u662f\u4e0d\u53d7\u5f71\u54cd\u7684\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">8.\u590d\u5408\u52a8\u4f5c\u7535\u4f4d (CAP) \u7684\u8bb0\u5f55<\/h2>\n\n\n\n<p>CAPs\u662f\u6839\u636e\u4e4b\u524d\u7684\u63cf\u8ff0\u4ece\u4f53\u5916\u5207\u7247\u51c6\u5907\u4e2d\u7684\u80fc\u80dd\u4f53\u8bb0\u5f55\u7684 (Reeves et al., 2005)\u3002\u7b80\u8981\u5730\u8bf4\uff0c\u5c0f\u9f20\u88ab\u5f02\u6c1f\u70f7\u9ebb\u9189\uff0c\u7136\u540e\u88ab\u5904\u6b7b\uff0c\u5927\u8111\u8fc5\u901f\u5207\u5272\u5230\u542b\u6709\u4ee5\u4e0b\u6210\u5206\u7684\u51b0\u51b7\u5207\u5272\u6eb6\u6db2\u4e2d\uff1a250 mM \u8517\u7cd6\uff0c25 mM D-\u8461\u8404\u7cd6\uff0c2.5 mM KCl\uff0c24 mM NaHCO\u2083\uff0c1.25 mM NaH\u2082PO\u2084\uff0c2.0 mM CaCl\u2082\uff0c1.5 mM MgSO\u2084 \u548c1.0 mM \u55b9\u5549\u9178 (pH 7.3\u20137.4)\u3002\u4f7f\u7528\u632f\u52a8\u5207\u7247\u673a (VT1200S, Leica) \u51c6\u5907400\u5fae\u7c73\u7684\u6574\u8111\u51a0\u72b6\u5207\u7247\u3002\u542b\u6709\u5b8c\u6574\u80fc\u80dd\u4f53\u7684\u8111\u7247\u88ab\u8f6c\u79fb\u5230\u586b\u5145\u4e86aCSF\u7684\u5ba4\u4e2d\uff0caCSF\u7ecf95%\u6c27\u548c5%\u4e8c\u6c27\u5316\u78b3\u901a\u6c14\uff0c\u5e76\u5305\u542b\u4ee5\u4e0b\u6210\u5206\uff1a124 mM NaCl\uff0c3 mM KCl\uff0c24 mM NaHCO\u2083\uff0c1.25 mM NaH\u2082PO\u2084\uff0c2 mM CaCl\u2082\uff0c1.0 mM MgSO\u2084\uff0c\u548c10 mM D-\u8461\u8404\u7cd6 (pH 7.3\u20137.4)\u3002\u5141\u8bb8\u5b83\u4eec\u572837\u00b0C\u4e0b\u6062\u590d30\u5206\u949f\uff0c\u7136\u540e\u79fb\u5230\u5ba4\u6e29\u4e0b\u81f3\u5c111\u5c0f\u65f6\u3002\u5207\u7247\u88ab\u8f6c\u79fb\u5230\u5ba4\u6e29\u4e0b\u7684\u6d78\u6ca1\u5ba4\u4e2d\uff0c\u5e76\u4f7f\u7528\u91cd\u529b\u704c\u6d41\u7cfb\u7edf\u8fdb\u884c\u6eb6\u6db2\u4ea4\u6362(2\u20133 ml\/min)\uff0c\u5728\u8bb0\u5f55\u671f\u95f4\u4f7f\u7528\u542b\u6c27\u7684aCSF\u704c\u6d41\u3002\u586b\u5145aCSF\u7684\u787c\u7845\u9178\u73bb\u7483\u7535\u6781(1.5-3 MV)\u88ab\u5b9a\u4f4d\u5728\u4e00\u4e2a\u534a\u7403\u7684\u80fc\u80dd\u4f53\u4e2d\u592e\uff0c\u901a\u8fc7\u653e\u7f6e\u5728\u53e6\u4e00\u4e2a\u534a\u7403\u7684\u80fc\u80dd\u4f53\u4e2d\u7684\u5b9a\u5236\u626d\u66f2\u954d\u94ec\u4e1d\u523a\u6fc0\u7535\u6781\u6765\u8bb0\u5f55\u901a\u8fc7\u7535\u523a\u6fc0\u8bf1\u53d1\u7684CAPs\u3002\u7535\u6781\u4e4b\u95f4\u7684\u8ddd\u79bb\u7ea6\u4e3a1.5 mm\u3002\u5e94\u7528\u4e0d\u540c\u7684\u523a\u6fc0\u5f3a\u5ea6(0\u20132 mA\uff0c21 \u6b65\u9aa4\uff0c200 ms\uff0c\u6bcf5\u79d2)\u6765\u8bf1\u53d1CAPs\u5e76\u521b\u5efa\u5f3a\u5ea6-\u53cd\u5e94\u56fe\u3002CAPs\u57281 kHz\u5904\u4f4e\u901a\u6ee4\u6ce2\u5e76\u4ee550 kHz\u6570\u5b57\u91c7\u6837\u3002\u6240\u6709\u6570\u636e\u4f7f\u7528Axopatch 200B\u653e\u5927\u5668\uff0cDigidata 1440A \u548cpClamp 10.6\u8f6f\u4ef6 (Molecular Devices) \u6570\u5b57\u5316\u3002\u4f7f\u7528Clampfit 10.6\u8f6f\u4ef6\u4ece\u5178\u578b\u7684\u8ff9\u7ebf\u5728\u7ebf\u76d1\u6d4b\u548c\u79bb\u7ebf\u5206\u6790\u6570\u636e\uff1b\u6bcf\u7ec46\u53ea\u5c0f\u9f20\uff1b\u4ece\u6bcf\u53ea\u5c0f\u9f20\u7684\u4e09\u5230\u56db\u7247\u8111\u7247\u4e2d\u5e73\u5747\u5f97\u5230N1\u3001N2 \u548c N2\/N1 \u6bd4\u503c\u3002\u7136\u540e\u4f7f\u7528\u5c0f\u9f20\u7684\u5e73\u5747\u503c\u8ba1\u7b97\u7ec4\u5747\u503c\u548c\u8bef\u5dee\u3002\u4ee3\u8868\u6027\u7684\u8ff9\u7ebf\u662f\u901a\u8fc7\u6700\u5927\u523a\u6fc0\u8bf1\u53d1\u7684\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">9.\u795e\u7ecf\u5143\u8ffd\u8e2a\u548c\u6811\u7a81\u68d8\u5206\u6790<\/h2>\n\n\n\n<p>\u6811\u7a81\u590d\u6742\u6027\u662f\u6839\u636e\u4e4b\u524d\u63cf\u8ff0\u8fdb\u884c\u786e\u5b9a\u7684\uff0c\u4f46\u8fdb\u884c\u4e86\u5c11\u91cf\u4fee\u6539 (Hao et al., 2016; Chunchai et al., 2018)\u3002\u7b80\u800c\u8a00\u4e4b\uff0c\u4f7f\u7528Thy1-YFP-H\u5c0f\u9f20\uff0c\u56e0\u4e3a\u7ea610\u201312%\u7684\u7b2cV\u5c42\u76ae\u5c42\u795e\u7ecf\u5143\u8868\u8fbeYFP\u5e76\u5141\u8bb8\u89c2\u5bdf\u5c0f\u7684\u795e\u7ecf\u7ed3\u6784 (Feng et al., 2000)\u3002\u5c0f\u9f20\u53d7\u4f24\u3001\u704c\u6ce8\u3001\u56fa\u5b9a\uff0c\u7136\u540e\u5927\u8111\u88ab\u5207\u621030\u5fae\u7c73\u539a\u7684\u76ae\u8d28\u5207\u7247\u3002\u4f7f\u7528Leica SP8\u5171\u7126\u663e\u5fae\u955c (Leica Biosystems) \u4ece\u76ae\u8d28\u7b2cIV\/V\u5c42\u83b7\u53d6z\u5806\u6808\u7684\u7cfb\u5217\u3002\u901a\u8fc7Imaris\u8f6f\u4ef67.0 (Oxford Instruments) \u5206\u6790\u795e\u7ecf\u5143\u5f62\u6001\u3002\u6bcf\u4e2a\u8111\u7247\u8bc4\u4f30\u4e09\u4e2a\u795e\u7ecf\u7ec6\u80de\uff0c\u6bcf\u53ea\u5c0f\u9f20\u8bc4\u4f30\u4e09\u4e2a\u8111\u7247\uff0c\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002\u5168\u795e\u7ecf\u5143\u88ab\u8ffd\u8e2a\uff0c\u4f7f\u7528Imaris\u8f6f\u4ef6\u8fdb\u884cSholl\u5206\u6790\u6765\u8bc4\u4f30\u8fc7\u7a0b\u7684\u5206\u652f\/\u590d\u6742\u6027\u3002\u4e0eSholl\u534a\u5f84\u76f8\u6bd4\uff0c\u4ea4\u70b9\u7684\u6570\u91cf\u88ab\u7ed8\u5236\uff0c\u5e76\u4ece3D\u7ed3\u6784\u8ba1\u7b97\u66f2\u7ebf\u4e0b\u9762\u79ef\u3002\u6b64\u5916\uff0c\u4e3a\u4e86\u786e\u5b9a\u6811\u7a81\u68d8\u5bc6\u5ea6\uff0c\u4f7f\u7528\u4e09\u4e2a\u4e09\u7ea7\u7247\u6bb5\u968f\u673a\u6d4b\u91cf\u6811\u7a81\u68d8\u5bc6\u5ea6\u3002\u6bcf10\u5fae\u7c73\u7684\u6811\u7a81\u6bb5\u91cf\u5316YFP+\u6811\u7a81\uff0c\u5e76\u4f7f\u7528Imaris\u8fdb\u4e00\u6b65\u5206\u7c7b\u4e3a\u8611\u83c7\u6216\u77ed\u7c97\u7684\u68d8\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p><strong>\u5927\u8111\u76ae\u5c42\u7684\u5206\u5c42<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\u56fe\u7247[1]-\u5403\u4e86\u5403\u4e86\" decoding=\"async\" src=\"https:\/\/cdn.ineuro.net\/cloudreve%2F2023%2F09%2F08%2F8bayYPE0_236253006_1_20211220070923255_wm.png\" \/><\/figure>\n\n\n\n<p>\u5927\u8111\u76ae\u5c42\u4e3b\u8981\u5206\u4e3a\u5206\u5b50\u5c42\u3001\u5916\u9897\u7c92\u5c42\u3001\u5916\u9525\u4f53\u5c42\u3001\u5185\u9897\u7c92\u5c42\u3001\u5185\u9525\u4f53\u3001\u591a\u5f62\u7ec6\u80de\u5c42\u3002\u5927\u8111\u76ae\u5c42\u76841-4\u5c42\u4e3b\u8981\u63a5\u53d7\u4f20\u5165\u7684\u51b2\u52a8\u3002<\/p>\n\n\n\n<p>\u76ae\u5c42\u7ec6\u80de\u7684\u7eb5\u5411\u67f1\u72b6\u6392\u5217\u6784\u6210\u5927\u8111\u76ae\u5c42\u7684\u6700\u57fa\u672c\u529f\u80fd\u7535\u4f4d\uff0c\u79f0\u4e3a\u611f\u89c9\u67f1\uff08sensory column\uff09\u3002\u8fd9\u79cd\u67f1\u72b6\u7ed3\u6784\u7684\u76f4\u5f84\u4e3a200-500\u03bcm\uff0c\u5782\u76f4\u8d70\u5411\u8111\u8868\u9762\uff0c\u8d2f\u7a7f\u6574\u4e2a\u516d\u5c42\u3002\u540c\u4e00\u67f1\u72b6\u7ed3\u6784\u5185\u7684\u795e\u7ecf\u5143\u90fd\u5177\u6709\u540c\u4e00\u79cd\u529f\u80fd\uff0c\u4f8b\u5982\u90fd\u5bf9\u540c\u4e00\u611f\u53d7\u91ce\u7684\u540c\u4e00\u7c7b\u578b\u611f\u89c9\u523a\u6fc0\u8d77\u53cd\u5e94\u3002\u5728\u540c\u4e00\u523a\u6fc0\u540e\uff0c\u8fd9\u4e9b\u795e\u7ecf\u5143\u53d1\u751f\u653e\u7535\u7684\u6f5c\u4f0f\u671f\u5f88\u63a5\u8fd1\uff0c\u4ec5\u76f8\u5dee2-4ms\uff1b\u8bf4\u660e\u5148\u6fc0\u6d3b\u7684\u795e\u7ecf\u5143\u4e0e\u540e\u6fc0\u6d3b\u7684\u795e\u7ecf\u5143\u4e4b\u95f4\u4ec5\u6709\u51e0\u4e2a\u795e\u7ecf\u5143\u63a5\u66ff\uff1b\u4ea6\u8bf4\u660e\u540c\u4e00\u67f1\u72b6\u7ed3\u6784\u5185\u795e\u7ecf\u5143\u8054\u7cfb\u73af\u8def\u53ea\u9700\u901a\u8fc7\u51e0\u4e2a\u795e\u7ecf\u5143\u63a5\u66ff\u5c31\u80fd\u5b8c\u6210\u3002\u4e00\u4e2a\u67f1\u72b6\u7ed3\u6784\u662f\u4e00\u4e2a\u4f20\u5165-\u4f20\u51fa\u4fe1\u606f\u6574\u5408\u5904\u7406\u5355\u4f4d\uff0c\u4f20\u5165\u51b2\u52a8\u5148\u8fdb\u5165\u7b2c\u56db\u5c42\uff0c\u5e76\u7531\u7b2c\u56db\u5c42\u548c\u7b2c\u4e8c\u5c42\u7ec6\u80de\u5728\u67f1\u5185\u5782\u76f4\u6269\u5e03\uff0c\u6700\u540e\u7531\u7b2c\u4e09\u3001\u7b2c\u4e94\u3001\u7b2c\u516d\u5c42\u53d1\u51fa\u4f20\u51fa\u51b2\u52a8\u79bb\u5f00\u5927\u8111\u76ae\u5c42\u3002\u7b2c\u4e09\u5c42\u7ec6\u80de\u7684\u6c34\u5e73\u7ea4\u7ef4\u8fd8\u6709\u6291\u5236\u76f8\u90bb\u7ec6\u80de\u67f1\u7684\u4f5c\u7528\uff1b\u56e0\u6b64\u4e00\u67f1\u53d1\u751f\u5174\u594b\u6d3b\u52a8\u65f6\uff0c\u5176\u76f8\u90bb\u7ec6\u80de\u67f1\u5c31\u53d7\u6291\u5236\uff0c\u5f62\u6210\u5174\u594b\u548c\u6291\u5236\u9576\u5d4c\u6a21\u5f0f\u3002\u8fd9\u79cd\u67f1\u72b6\u7ed3\u6784\u7684\u5f62\u6001\u529f\u80fd\u7279\u70b9\uff0c\u5728\u7b2c\u4e8c\u611f\u89c9\u533a\u3001\u89c6\u533a\u3001\u542c\u533a\u76ae\u5c42\u548c\u8fd0\u52a8\u533a\u76ae\u5c42\u4e2d\u4e5f\u4e00\u6837\u5b58\u5728\u3002<\/p>\n<\/blockquote>\n\n\n\n<h2 class=\"wp-block-heading\">10.\u8fd0\u52a8\u6d3b\u52a8\u7684Rotarod\u548c\u60ac\u6302\u7ebf\u8bc4\u4f30<\/h2>\n\n\n\n<p>\u4e4b\u524d\u63cf\u8ff0\u7684\u52a0\u901fRotarod\u6d4b\u8bd5\u8fdb\u884c\u4e86 (Fenn et al., 2014)\u3002\u7b80\u800c\u8a00\u4e4b\uff0c\u786e\u5b9a\u4ece\u52a0\u901fJrotarod\u4e0a\u6389\u843d\u7684\u6f5c\u4f0f\u671f\u3002\u65cb\u8f6c\u901f\u5ea6\u4ece4 rpm\u5f00\u59cb\uff0c\u52a0\u901f\u523040 rpm\u8d85\u8fc7200\u79d2\u3002\u5c0f\u9f20\u6bcf\u5929\u6709\u4e09\u6b21\u5c1d\u8bd5\uff0c\u8fd9\u4e9b\u88ab\u5e73\u5747\u3002\u57f9\u8bad\u662f\u5728mFPI\u4e4b\u524d\u76843\u5929\uff0c\u6700\u540e\u4e00\u5929\u8ba1\u4e3a\u57fa\u7ebf\u6027\u80fd\u3002\u6d4b\u8bd5\u662f\u5728TBI\u540e\u76841dpi\u548c\u6bcf\u5468\u4e00\u6b21\u3002\u50cf\u4e4b\u524d\u63cf\u8ff0\u7684\u90a3\u6837\u8fdb\u884c\u4e86\u6c34\u5e73\u6761\u6746\u60ac\u6302\u6d4b\u8bd5 (Deacon, 2013)\u3002\u7b80\u800c\u8a00\u4e4b\uff0c\u5c06\u524d\u722a\u653e\u5728\u4e00\u4e2a6\u6beb\u7c73\u76f4\u5f84\u7684\u786c\u7ebf\u4e0a\uff0c\u8fd9\u4e2a\u7ebf\u88ab\u5347\u9ad8\u5728\u4e00\u4e2a\u57ab\u5b50\u4e0a\uff0c\u5e76\u786e\u5b9a\u5c0f\u9f20\u9003\u5230\u4e00\u4e2a\u8ddd\u79bb20\u5398\u7c73\u7684\u5e73\u53f0\u4e0a\u7684\u6f5c\u4f0f\u671f\u3002\u5728\u8fd9\u6b21\u6d4b\u8bd5\u4e2d\uff0c\u6ca1\u6709\u5c0f\u9f20\u4ece\u7ebf\u4e0a\u6389\u4e0b\u6765\uff1b\u6bcf\u4e2a\u5b9e\u9a8c\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">11.\u65b0\u7269\u4f53\u4f4d\u7f6e(NOL)\u548c\u8bc6\u522b<\/h2>\n\n\n\n<p>\u4e4b\u524d\u63cf\u8ff0\u7684NOL\u548c\u8bc6\u522b\u8fdb\u884c\u4e86 (Denninger et al., 2018) \u4f46\u8fdb\u884c\u4e86\u5c11\u91cf\u4fee\u6539\u3002\u8fd9\u4e9b\u6d4b\u8bd5\u6d89\u53ca\u6bcf\u4e2a\u5206\u969424\u5c0f\u65f6\u7684\u56db\u4e2a10\u5206\u949f\u9636\u6bb5\uff1a\u4e60\u60ef\u5316(\u6ca1\u6709\u7269\u4f53)\uff0c\u9002\u5e94(\u4e24\u4e2a\u7269\u4f53)\uff0c\u4f4d\u7f6e(\u4e24\u4e2a\u7269\u4f53\uff0c\u4e00\u4e2a\u65b0\u4f4d\u7f6e)\u548c\u8bc6\u522b(\u4e24\u4e2a\u7269\u4f53\uff0c\u6709\u4e00\u4e2a\u65b0\u7269\u4f53)\u3002\u8bd5\u9a8c\u88ab\u5f55\u5236\uff0c\u5e76\u901a\u8fc7\u4e00\u4e2a\u76f2\u5ba1\u8005\u6d4b\u91cf\u6bcf\u4e2a\u7269\u4f53\u7684\u8c03\u67e5\u65f6\u95f4\u3002\u5bf9\u4e8e\u4f4d\u7f6e\u548c\u8bc6\u522b\u8bd5\u9a8c\uff0c\u8ba1\u7b97\u9274\u522b\u6307\u6570\u5982\u4e0b\uff1a[(\u65b0\u65f6\u95f4 &#8211; \u719f\u6089\u65f6\u95f4)\/\u603b\u65f6\u95f4] * 100\uff1b\u6bcf\u4e2a\u5b9e\u9a8c\u7ec410\u53ea\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">12.\u5b9e\u9a8c\u8bbe\u8ba1\u548c\u7edf\u8ba1\u5206\u6790<\/h2>\n\n\n\n<p>\u6bcf\u4e2a\u5b9e\u9a8c\u4e2d\u7684\u5c0f\u9f20\u6570\u91cf\u5305\u62ec\u5206\u6790\u7684\u5207\u7247\/\u7ec6\u80de\/\u56fe\u50cf\u6570\u91cf\u90fd\u5305\u542b\u5728\u4e0a\u8ff0\u6bcf\u4e2a\u5b9e\u9a8c\u7684\u65b9\u6cd5\u4e2d\u3002\u4f7f\u7528DESeq2\u6216CellRanger\u5206\u522b\u63cf\u8ff0\u4e0a\u8ff0\u7684NanoString\u548c\u5355\u7ec6\u80deRNA-\u6d4b\u5e8f(scRNAseq)\u7684\u7edf\u8ba1\u5206\u6790\u3002\u7528\u4e8e\u7ec4\u7ec7\u5b66\u3001\u884c\u4e3a\u548c\u7535\u751f\u7406\u6570\u636e\u7684ANOVA\u7684IBM SPSS\u7edf\u8ba1(\u7248\u672c24)\u3002\u4f7f\u7528\u9002\u5f53\u7684\u65b9\u6cd5\u786e\u5b9a\u4e3b\u6548\u5e94\u548c\u56e0\u7d20\u4e4b\u95f4\u7684\u4ea4\u4e92\u3002\u5f53\u786e\u5b9a\u4e3b\u6548\u5e94\u548c\/\u6216\u4ea4\u4e92\u65f6\uff0c\u7528\u4e8e\u4e8b\u540e\u5206\u6790\u7684\u6700\u5c0f\u5e73\u65b9\u5dee (LSD)\uff0c\u5e76\u8ba4\u4e3ap &lt; 0.05\u662f\u7edf\u8ba1\u5b66\u4e0a\u663e\u8457\u7684\u3002<\/p>\n\n\n\n<h1 class=\"wp-block-heading\">\u4e09\u3001\u7814\u7a76\u7ed3\u679c<\/h1>\n\n\n\n<h2 class=\"wp-block-heading\">1.\u4f9d\u8d56\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u548c\u4e0d\u4f9d\u8d56\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u76ae\u5c42\u57fa\u56e0\u8868\u8fbe\u57281\u30017\u300130\u5929\u540e\u7684\u635f\u4f24\u4e2d\u53d1\u751f\u5dee\u5f02\u6027\u53d8\u5316<\/h2>\n\n\n\n<p>\u7814\u7a76\u4eba\u5458\u4e4b\u524d\u62a5\u9053\uff0c\u901a\u8fc7CSF1R\u62ee\u6297\u5242PLX5622\u6d88\u9664\u5c0f\u80f6\u8d28\u7ec6\u80de\u53ef\u4ee5\u9006\u8f6c\u708e\u75c7\u76f8\u5173\u57fa\u56e0\u7684mRNA\u8868\u8fbe\uff0c\u5e76\u51cf\u5c11\u76ae\u5c427\u5929\u540e\u7684\u635f\u4f24\u4e2d\u5f62\u6210\u72ec\u7279\u7684\u68d2\u72b6\u5c0f\u80f6\u8d28\u7ec6\u80de(Witcher\u7b49\uff0c2018)\u3002\u5728\u8fd9\u91cc\uff0c\u7814\u7a76\u4eba\u5458\u8bd5\u56fe\u786e\u5b9a\u5c0f\u80f6\u8d28\u7ec6\u80de\u5bf9\u6025\u6027(1 dpi)\u3001\u4e9a\u6025\u6027(7 dpi)\u548c\u6162\u6027\u65f6\u95f4\u70b9(30 dpi)\u76ae\u5c42\u795e\u7ecf\u75c5\u7406\u5b66\u7684\u8d21\u732e\u3002\u901a\u8fc7\u53e3\u670dPLX5622 14\u5929\u6765\u6d88\u9664\u5c0f\u80f6\u8d28\u7ec6\u80de\uff0c\u7136\u540e\u5728\u5b9e\u9a8c\u671f\u95f4\u7ef4\u6301\u5b9e\u9a8c\u996e\u98df(Veh\u6216PLX5622)\u3002\u5728\u6bcf\u4e2a\u7ec8\u70b9\uff0c\u6536\u96c6\u5927\u8111\uff0c\u5207\u7247\uff0c\u5e76\u7528\u6297Iba1\u6807\u8bb0\u3002\u5982\u9884\u671f(Elmore\u7b49\uff0c2014;Witcher\u7b49\uff0c2018)\uff0c\u5728\u57fa\u7ebf\uff0cPLX5622\u7ed9\u836f\u540eIba11\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6570\u91cf\u663e\u8457\u51cf\u5c11(F(1,31)=1658, p&lt;0.001; \u56fe1B,C) \u548c\u57281\u30017\u3001\u621630 dpi (F(3,31) = 4.3, p = 0.015)\u3002\u5207\u7247\u4e0eP2RY12\u5171\u6807\uff0c\u8d85\u8fc799%\u7684Iba1+\u7ec6\u80de\u4e5f\u662fP2RY121+(\u6570\u636e\u672a\u663e\u793a)\u3002\u603b\u4f53\u6765\u8bf4\uff0c\u5728PLX5622\u7ed9\u836f\u671f\u95f4\u5c0f\u80f6\u8d28\u7ec6\u80de\u88ab\u6d88\u8017\u3002<\/p>\n\n\n\n<p>\u5728\u53e6\u4e00\u9879\u5b9e\u9a8c\u4e2d\uff0c\u6536\u96c6\u5927\u8111\u5e76\u51b7\u51bb\u5207\u7247\uff0c\u7528\u6297-CD11b\u6807\u8bb0\u4ee5\u53ef\u89c6\u5316\u5c0f\u80f6\u8d28\u7ec6\u80de\u3002\u6839\u636e\u5728\u5f15\u5bfc\u5207\u7247\u4e0a\u53d1\u73b0\u7684\u53cd\u5e94\u6027\u6216\u6746\u72b6\u5c0f\u80f6\u8d28\u7ec6\u80de\uff0c\u5728\u51b7\u51bb\u7684\u540c\u65f6\u5bf9\u76f8\u90bb\u539a\u5207\u7247\u7684\u76f8\u5e94\u76ae\u5c42\u533a\u57df\u8fdb\u884c\u663e\u5fae\u89e3\u5256 \u3002\u4f24\u540e\u5fae\u5207\u76ae\u5c42\u4e2d\u4e0e\u795e\u7ecf\u75c5\u7406\u5b66\u76f8\u5173\u7684\u57fa\u56e0\u8868\u8fbe\u5206\u6790(NanoString\u795e\u7ecf\u75c5\u7406\u5b66nCounter\u9762\u677f\uff0c760\u4e2a\u57fa\u56e0\u52a030\u4e2a\u81ea\u5b9a\u4e49\u57fa\u56e0)\u663e\u793a\uff0c\u57fa\u56e0\u7684\u5dee\u5f02\u8868\u8fbe\u53d7\u5230\u4f24\u5bb3\u3001\u65f6\u95f4\u548c\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u7684\u5f71\u54cd(\u56fe1D)\u3002\u4f8b\u5982\uff0c1 dpi\u65f6\u6709114\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0(76\u4e2a\u589e\u52a0\u548c38\u4e2a\u51cf\u5c11)\u3002\u57287 dpi\u670961\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0(40\u4e2a\u589e\u52a0\u548c21\u4e2a\u51cf\u5c11)\uff0c\u572830 dpi\u670929\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0(18\u4e2a\u589e\u52a0\u548c11\u4e2a\u51cf\u5c11)\u3002\u56fe1D\u7a81\u51fa\u4e86\u90a3\u4e9b\u4e0ePLX\u72ec\u7acb(\u767d\u8272\u6761)\u6216\u4f9d\u8d56\u4e8ePLX (p&lt;0.05, \u9ed1\u8272\u6761)\u7684TBI\u8bf1\u5bfc\u7684\u57fa\u56e0\u3002\u57281 dpi\uff0c\u53ea\u670933%\u7684\u7531TBI\u5f71\u54cd\u7684\u57fa\u56e0\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u88ab\u9006\u8f6c\u3002\u88ab\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017(PLX5622)\u5f71\u54cd\u7684TBI\u8bf1\u5bfc\u57fa\u56e0\u7684\u8d21\u732e\u57287 dpi\u589e\u52a0\u523066%\uff0c\u572830 dpi\u589e\u52a0\u523080%\u3002\u7efc\u4e0a\u6240\u8ff0\uff0cTBI\u540e\u7684\u6025\u6027\u57fa\u56e0\u8868\u8fbe\u4e3b\u8981\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u65e0\u5173\uff0c \u800c\u795e\u7ecf\u75c5\u7406mRNA\u8868\u8fbe\u7684\u6301\u7eed\u53d8\u5316\u4f9d\u8d56\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u3002<\/p>\n\n\n\n<p>\u63a5\u4e0b\u6765\uff0cIPA\u88ab\u7528\u6765\u9274\u5b9a\u8fd9\u4e9b\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u7ecf\u5178\u4fe1\u53f7\u901a\u8def\u548c\u4e0a\u6e38\u8c03\u8282\u56e0\u5b50\u3002IPA\u7684\u539f\u59cb\u8f93\u51fa\u5305\u62ec\u5728\u6269\u5c55\u6570\u636e\u56fe1-3\u3002\u7136\u540e\u4f7f\u7528IPA\u6bd4\u8f83\u5206\u6790\u6765\u6bd4\u8f83\u4f24\u540e\u65f6\u95f4\u70b9(1, 7, 30 dpi)\u548c\u6cbb\u7597\u6761\u4ef6(Veh, PLX5622; \u56fe1E)\u7684\u901a\u8def\u53d8\u5316\u3002\u4e0e\u795e\u7ecf\u708e\u75c7\u76f8\u5173\u7684\u7ecf\u5178\u901a\u8def\u57281, 7, \u548c30 dpi\u88ab\u7ef4\u6301\u3002\u5728\u6025\u6027\u548c\u4e9a\u6025\u6027\u65f6\u95f4\u70b9(1\u548c7 dpi)\u589e\u52a0\u7684\u5176\u4ed6\u663e\u8457\u7684\u7ecf\u5178\u901a\u8def\u5305\u62ecp38\u4fe1\u53f7\u3001NO\u548cROS\u4ea7\u751f\u4ee5\u53caHMGB1\u4fe1\u53f7\u3002\u5728\u6025\u6027\u548c\u4e9a\u6025\u6027\u4f24\u5bb3\u7684\u4e24\u4e2a\u9636\u6bb5\uff0cTBI\u540eLXR\/RXR\u901a\u8def\u88ab\u6291\u5236\u3002\u8fd9\u4e9bTBI\u7684\u901a\u8def\u7ea7\u6548\u5e94\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u8017\u88ab\u7f13\u89e3\u3002<\/p>\n\n\n\n<p>IPA\u4e0a\u6e38\u8c03\u63a7\u56e0\u5b50\u5206\u6790\uff08p &lt; 0.05\uff1b\u7edd\u5bf9z\u5f97\u5206 &gt; 2\uff09\u63ed\u793a\u4e86TBI\u548c\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u7684\u76f8\u4f3c\u6548\u5e94\u3002\u8fd9\u79cd\u5206\u6790\u9884\u6d4b\u53ef\u80fd\u89e3\u91ca\u57fa\u56e0\u8868\u8fbe\u89c2\u5bdf\u6a21\u5f0f\u7684\u4fe1\u53f7\u901a\u8def\u3002\u5728\u6025\u6027\u548c\u4e9a\u6025\u6027\u65f6\u95f4\u70b9\uff081\u548c7 dpi\uff09\u7684\u8868\u8fbe\u6a21\u5f0f\u4e0e\u5347\u9ad8\u7684\u7ec6\u80de\u56e0\u5b50\uff08IL-6, TNF, IL-1b\uff09\u548c\u5e72\u6270\u7d20\uff08IFNa, IFNb, IFNg\uff09\u4fe1\u53f7\u4e00\u81f4\u3002\u5176\u4e2d\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0cTNF\u76f8\u5173\u57fa\u56e0\u572830 dpi\u4ecd\u7136\u589e\u9ad8\u3002\u57281\u548c7 dpi\uff0c\u6709\u4e00\u4e2a\u4e0eTBI\u5173\u8054\u7684APOE\u548cSOCS1\u901a\u8def\u7684\u6291\u5236\u3002\u603b\u4f53\u6765\u8bf4\uff0c\u8fd9\u4e9bTBI\u540e\u7684\u8868\u8fbe\u6a21\u5f0f\u5728\u6ca1\u6709\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u5c0f\u9f20\u4e2d\u4e0d\u5b58\u5728\uff0c\u5e76\u4e14\u4e0e\u5bf9\u7167\u7ec4\u663e\u793a\u76f8\u53cd\u7684\u8868\u8fbe\u6a21\u5f0f\uff08\u5982\u56fe1E\uff0c\u5e95\u90e8\u9762\u677f\u6240\u793a\uff09\u3002<\/p>\n\n\n\n<p>\u6709\u548c\u6ca1\u6709PLX5622\u7684\u60c5\u51b5\u4e0b\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u8bc1\u5b9e\u4e86\u7ec4\u7ec7\u5b66\u4e0a\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u7684\u8bc1\u636e\u3002\u56fe1F\u663e\u793a\u4e86\u53d7TBI\u548cPLX\u5f71\u54cd\u7684\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u5173\u7684\u57fa\u56e0\u7684\u70ed\u56fe\uff0c\u5206\u522b\u4e3a1\u30017\u621630 dpi\u3002\u4f8b\u5982\uff0cPLX5622\u5bf9\u5305\u62ecTmem119\u3001Cx3cr1\u3001Csf1r\u3001C1qc\u3001Trem2\u548cItgam\uff08CD11b\uff09\u5728\u5185\u7684\u5927\u591a\u6570\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u5173\u7684\u57fa\u56e0\u4ea7\u751f\u4e86\u4e3b\u8981\u5f71\u54cd\uff08p\uff0c0.05\uff09\u3002\u4e0eTBI\uff08p\uff0c0.05\uff09\u76f8\u5173\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u5173\u57fa\u56e0Il10ra\u3001Gpr84\u3001Cd68\u3001Cd14\u3001Ccr5\u3001Cd33\u548cNlrp3\u5728TBI\u524d\u5df2\u7ecf\u51cf\u5c11\u7684\u5c0f\u9f20\u4e2d\u53d7\u5230\u4e86\u6291\u5236\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u4e00\u4e9b\u5176\u4ed6\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\/\u9ad3\u6837\u7ec6\u80de\u76f8\u5173\u7684\u57fa\u56e0\uff0c\u5982Ccr7\u3001Il1b\u3001H2-Eb1\u548cCcl2\uff0c\u5728\u4e0d\u8003\u8651PLX5622\u7684\u60c5\u51b5\u4e0b\u4e5f\u53d7\u5230TBI\uff08p\uff0c0.05\uff09\u7684\u5f71\u54cd\u3002\u4e0ePLX-Con\u5c0f\u9f20\u76f8\u6bd4\uff0cPLX-TBI\u5c0f\u9f20\u572830 dpi\u65f6\u8868\u73b0\u51fa\u4e86\u4e00\u4e9b\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u5173\u7684\u57fa\u56e0\u7684\u5347\u9ad8\uff0c\u5305\u62ecCd68\u3001Cd14\u548cTrem2\u3002\u8fd9\u53ef\u80fd\u4e0ePLX-TBI\u5c0f\u9f20\u572830 dpi\u65f6\u8111\u5185\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u5bf9\u6269\u589e\u6709\u5173\uff08\u56fe1C\uff09\u3002\u603b\u7684\u6765\u8bf4\uff0c\u5927\u591a\u6570\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u5173\u7684\u708e\u75c7\u53cd\u5e94\u5728TBI\u524d\u540e\u5c0f\u80f6\u8d28\u7ec6\u80de\u88ab\u6e05\u9664\u65f6\u57281 dpi\u88ab\u963b\u65ad\uff0c\u800c\u57287 dpi\u548c30 dpi\u65f6\u4fdd\u6301\u4f4e\u6c34\u5e73\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">2.\u5728\u76ae\u8d28\u4e2d\u7684\u708e\u75c7mRNA\u8868\u8fbe\u968f\u77401\u30017\u548c30 dpi\u7684\u8fdb\u5c55\u800c\u53d1\u5c55<\/h2>\n\n\n\n<p>\u4e0e\u56fe1\u4e2d\u5448\u73b0\u7684\u6570\u636e\u76f8\u5173\uff0c\u56fe2A\u4e2d\u7684\u70ed\u56fe\u7a81\u51fa\u4e86TBI\u548c\u65f6\u95f4\u5bf9\u4e0e\u795e\u7ecf\u75c5\u7406\u5b66\u76f8\u5173\u7684\u57fa\u56e0\u7684\u4ea4\u4e92\u4f5c\u7528\uff0c\u90e8\u5206\u53cd\u6620\u51fa\u57281\uff08\u9876\u90e8\uff09\u30017\uff08\u4e2d\u90e8\uff09\u548c30\uff08\u5e95\u90e8\uff09dpi\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff08p &lt; 0.05\uff09\u3002\u56fe2B\u53cd\u6620\u4e86\u5728\u591a\u4e2a\u65f6\u95f4\u70b9\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u3002\u603b\u4f53\u4e0a\uff0c\u4f24\u5bb3\u3001\u65f6\u95f4\u548c\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\uff08PLX5622\uff09\u90fd\u6709\u5f71\u54cd\u3002\u76ae\u8d28\u4e2d\u5dee\u5f02mRNA\u8868\u8fbe\u7684\u6700\u9ad8\u6c34\u5e73\u662f\u57281 dpi\u3002\u6025\u6027\u671f\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0cTBI\u589e\u52a0\u4e86\u51e0\u79cd\u4e0e\u708e\u75c7\u76f8\u5173\uff08Cd14\u3001Cd68\u3001Gpr84\u3001Myd88\u3001Tnf\uff09\u3001\u5e72\u6270\u7d20\u76f8\u5173\uff08Irf7\u3001Ifi2712a\uff09\u548c\u8d8b\u5316\u56e0\u5b50\u76f8\u5173\uff08Ccl12\u3001Ccl3\u3001Cxcl10\u3001Cxcl16\uff09\u7684\u8f6c\u5f55\u7269\uff08p &lt; 0.05\uff09\u3002\u8fd9\u4e9b\u4e0eTBI\u76f8\u5173\u76841 dpi\u7684\u589e\u52a0\u88abPLX5622\u51cf\u5f31\uff08p &lt; 0.05\uff09\u3002\u5c3d\u7ba1\u5982\u6b64\uff0c\u9ad8\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u8fbe\uff0866%\uff1b\u56fe1D\uff09\u4e0d\u53d7PLX\u7684\u5f71\u54cd\u3002\u4f8b\u5982\uff0cTBI\u589e\u52a0\u4e86Icam1\u3001Ptgs2\u3001Ccl2\u3001Cd44\u3001Gfap\u3001Atf3\u3001Osmr\u3001Nex\u3001\u80f6\u539f\u7ec4\u5206\uff08Col4a1\u3001Col4a2\u548cCol6a\uff09\u548c\u57fa\u8d28\u91d1\u5c5e\u86cb\u767d\u9176\uff08Mmp12\u3001Mmp14\u548cMmp19\uff09\u3002\u8fd9\u4e9b\u57fa\u56e0\u57281 dpi\u7684\u589e\u52a0\u4e0d\u53d7\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u7684\u5f71\u54cd\u3002\u56e0\u6b64\uff0c\u76ae\u8d28\u4e2d1 dpi\u7684mRNA\u5dee\u5f02\u8868\u8fbe\u5927\u90e8\u5206\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u65e0\u5173\u3002<\/p>\n\n\n\n<p>\u7136\u800c\uff0c\u968f\u7740\u65f6\u95f4\u7684\u63a8\u79fb\uff0c\u53d7TBI\u548c\u5c0f\u80f6\u8d28\u7ec6\u80de\u5f71\u54cd\u7684\u57fa\u56e0\u6570\u91cf\u589e\u52a0\u3002\u8fd9\u5728\u6309\u65f6\u95f4\u7ec4\u7ec7\u7684\u70ed\u56fe\u4e2d\u5f88\u660e\u663e\uff08\u56fe2A\uff09\u3002\u57287\u548c30 dpi\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u5728\u56fe2C\u3001D\u4e2d\u603b\u7ed3\uff0cBarres\u5b9e\u9a8c\u5ba4\u7684\u5927\u8111RNA-seq Mus musculus \uff08www.brainrnaseq.org)\u6570\u636e\u96c6\u88ab\u7528\u6765\u8fd1\u4f3c\u8868\u793a\u90a3\u79cd\u7ec6\u80de\u7c7b\u578b\u8868\u8fbe\u4e86\u54ea\u79cd\u57fa\u56e0\u3002\u57287 dpi\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u51e0\u79cd\u4e0e\u708e\u75c7\u76f8\u5173\uff08Cd14\u3001Cd68\u3001Gpr84\u3001Clec7a\u3001Itgax\u3001Tlr4\u3001Trem2\uff09\u3001\u5e72\u6270\u7d20\u76f8\u5173\uff08Irf7\u3001Ifi2712a\uff09\u548c\u8d8b\u5316\u56e0\u5b50\u76f8\u5173\uff08Ccl12\u3001Ccl3\u3001Cxcl10\u3001Cxcl16\uff09\u7684\u8f6c\u5f55\u7269\u589e\u52a0\uff08p &lt; 0.05\uff09\uff0c\u5e76\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u8017\u800c\u9006\u8f6c\uff08p &gt; 0.05\uff09\u3002\u76ae\u8d28\u4e2d\u8fd8\u6709\u51e0\u79cd\u57287 dpi\uff08p &lt; 0.05\uff09\u51cf\u5c11\u7684\u57fa\u56e0\uff0c\u5305\u62ec\u4e0e\u795e\u7ecf\u5143\u76f8\u5173\u7684\u57fa\u56e0\uff08Adcy8\u3001Cxc3rl\u3001Drd2\u3001Htr1a\u3001Ngf\u3001Slc18a3\u548cTrpv1\uff09\uff0c\u8fd9\u4e9b\u57fa\u56e0\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u8017\u800c\u88ab\u963b\u6b62\uff08p &gt; 0.05\uff09\u3002<\/p>\n\n\n\n<p>\u572830 dpi\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u51e0\u79cd\u4e0e\u5148\u5929\u514d\u75ab\u76f8\u5173\u7684\u8f6c\u5f55\u7269\uff08Cd14\u3001Cd68\u3001Gpr84\u3001Itgax\u3001Tlr4\u3001Trem2\uff09\u56e0TBI\u800c\u589e\u52a0\uff08p &lt; 0.05\uff09\uff0c\u5e76\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u8017\u800c\u9006\u8f6c\uff08p &gt; 0.05\uff09\u3002\u76ae\u8d28\u4e2d\u8fd8\u6709\u51e0\u79cd\u572830 dpi\uff08p &lt; 0.05\uff09\u51cf\u5c11\u7684\u57fa\u56e0\uff0c\u5305\u62ec\u4e0e\u795e\u7ecf\u5143\u76f8\u5173\u7684\u8f6c\u5f55\u7269\uff08Avp\u3001Drd1\u3001Drd2\uff09\uff0c\u8fd9\u4e9b\u51cf\u5c11\u518d\u6b21\u901a\u8fc7TBI\u524d\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u8017\u800c\u9006\u8f6c\uff08p &gt; 0.05\uff09\u3002<\/p>\n\n\n\n<p>\u572830 dpi\u7684\u53e6\u4e00\u4e2a\u503c\u5f97\u6ce8\u610f\u7684\u89c2\u5bdf\u662f\uff0c\u4e0eVeh-TBI\u76f8\u6bd4\uff0cTBI-PLX\u7ec4\u4e2d\u6709\u51e0\u79cd\u57fa\u56e0\u589e\u5f3a\u3002\u8fd9\u4e9b\u6570\u636e\u53ef\u80fd\u8868\u660e\uff0c\u5f53\u5c0f\u80f6\u8d28\u7ec6\u80de\u88ab\u6d88\u8017\u540e\uff0cTBI\u540e\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u6709\u4e86\u8865\u507f\/\u6269\u5f20\u3002\u4e3a\u4e86\u652f\u6301\u8fd9\u4e00\u89c2\u70b9\uff0c\u5728PLX-TBI\u7ec4\u4e0eVeh-TBI\u7ec4\u76f8\u6bd4\uff0cAldh1l1\u3001Cd44\u3001Gfap\u548cAqp4\u7684\u76f8\u5bf9mRNA\u8868\u8fbe\u66f4\u9ad8\uff08p &lt; 0.05\uff1b\u56fe2D\uff09\u3002\u6269\u5c55\u6570\u636e\u8fd8\u663e\u793a\uff0c\u5728TBI-PLX\u7ec4\u4e0eTBI-Con\u7ec430 dpi\uff08\u6269\u5c55\u6570\u636e\u56fe1-1\uff09\u4e2d\uff0cGFAP\u6807\u8bb0\u589e\u52a0\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">3.TBI\u540e\u76ae\u8d28\u7ec6\u80de\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u3002\u76ae\u8d28mRNA\u5206\u6790\u663e\u793a\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u5bf9\u4e8e\u4f24\u540e\u6301\u7eed\u7684\u795e\u7ecf\u708e\u75c7\u9014\u5f84\u6709\u7740\u5f3a\u70c8\u7684\u8d21\u732e\u3002<\/h2>\n\n\n\n<p>\u8fd9\u4e9b\u6570\u636e\u8fd8\u8868\u660e\uff0cTBI\u540e\u4e9a\u6025\u6027\u548c\u6162\u6027\u65f6\u95f4\u70b9\u7684\u6301\u7eed\u708e\u75c7\u53ef\u80fd\u5f71\u54cd\u5468\u56f4\u7684\u795e\u7ecf\u5143\u3001\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u548c\u5185\u76ae\u7ec6\u80de\u3002\u4e3a\u4e86\u8c03\u67e5\u8fd9\u4e00\u5047\u8bbe\uff0c\u91c7\u7528\u4e86\u5355\u7ec6\u80de\u6d4b\u5e8f\u65b9\u6cd5\u6765\u786e\u5b9a\u4f24\u540e7\u5929\u76ae\u8d28\u5185\u6709\u65e0\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u7684\u7ec6\u80de\u7c7b\u578b\u7279\u5f02\u6027\u57fa\u56e0\u8868\u8fbe\uff08\u56fe3A\uff09\u3002\u4ece\u663e\u5fae\u89e3\u5256\u7684\u76ae\u8d28\u4e2d\u4ea7\u751f\u7684\u6c47\u805a\u7684\u5355\u7ec6\u80de\u60ac\u6d6e\u6db2\uff0c\u4f7f\u752810x Genomics\u6d41\u7a0b\u8fdb\u884c\u5355\u7ec6\u80demRNA\u6d4b\u5e8f\u3002\u57fa\u4e8e\u7ec6\u80de\u9009\u62e9\u6027\u57fa\u56e0\u8868\u8fbe\u7684\u805a\u7c7b\u53ef\u4ee5\u660e\u663e\u5730\u770b\u5230\u4e0d\u540c\u7684\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u7ec6\u80de\u7c7b\u578b\uff08\u56fe3B,C\uff09\u3002\u4f8b\u5982\uff0c<strong>\u5c0f\u80f6\u8d28\u7ec6\u80de\uff08Tmem119\uff09\u3001\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\uff08Gja1\uff09\u3001\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\uff08Plp1\uff09\u3001\u795e\u7ecf\u5143\uff08Meg3\uff09\u548c\u5185\u76ae\u7ec6\u80de\uff08Ly6c1\uff09\u90fd\u5f62\u6210\u4e86\u4e0d\u540c\u7684\u7c07\uff08\u56fe3C\uff09<\/strong>\u3002\u56fe3D\u4e2d\u7684\u70b9\u56fe\u548c\u6269\u5c55\u6570\u636e\u56fe3-1\u8868\u793a\u4e86\u6240\u670936,000\u4e2a\u6d4b\u5e8f\u7ec6\u80de\u5728\u6240\u6709\u56db\u4e2a\u5b9e\u9a8c\u7ec4\uff08Veh-Con\u3001Veh-TBI\u3001PLX-Con\u3001PLX-TBI\uff09\u4e2d\u7684\u5206\u5e03\u3002\u6269\u5c55\u6570\u636e\u56fe3-1\u7a81\u51fa\u663e\u793a\u4e86Veh\u5904\u7406\u7ec4\u548cPLX\u5904\u7406\u7ec4\u4e4b\u95f4\u5c0f\u80f6\u8d28\u7ec6\u80de\u6570\u91cf\u7684\u5dee\u5f02\u3002\u6b64\u5916\uff0c\u5bf9\u5c0f\u80f6\u8d28\u7ec6\u80de\uff0812,181\u7ec6\u80de\uff09\u548c\u795e\u7ecf\u5143\uff082878\u7ec6\u80de\uff09\u79cd\u7fa4\u8fdb\u884c\u4e86\u5b50\u805a\u7c7b\u4ee5\u8fdb\u884c\u8fdb\u4e00\u6b65\u5206\u6790\u3002\u5b50\u805a\u7c7b\u7684\u70b9\u56fe\u663e\u793a\u4e86\u5c0f\u80f6\u8d28\u7ec6\u80de\uff08\u56fe3E\uff09\u548c\u795e\u7ecf\u5143\uff08\u56fe3F\uff09\u7684\u7ec6\u80de\u5206\u5e03\u60c5\u51b5\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\u56fe\u7247[2]-\u5403\u4e86\u5403\u4e86\" decoding=\"async\" src=\"https:\/\/cdn.ineuro.net\/cloudreve%2F2023%2F09%2F08%2F5GUTLjD1_wx2-1.png\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">4.\u521b\u4f24\u76f8\u5173\u7684mRNA\u7279\u5f81\u548cTBI\u540e\u76ae\u8d28\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e2d\u589e\u52a0\u7684type-1\u5e72\u6270\u7d20\u4fe1\u53f7<\/h2>\n\n\n\n<p>\u9996\u5148\uff0c<strong>\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u5b50\u805a\u7c7b\u63ed\u793a\u4e8610\u4e2a\uff080-9\uff09\u7531\u6bcf\u4e2a\u7ec6\u80de\u7684\u8f6c\u5f55\u7ec4\u786e\u5b9a\u7684\u660e\u663e\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\u7c07<\/strong>\uff08\u56fe4A\uff09\u3002\u56fe4B\u663e\u793a\u4e86\u6bcf\u4e2a\u7c07\u4e2d\u7684\u7ec6\u80de\u603b\u6570\uff0c\u4ee5\u53ca\u6765\u81ea\u6bcf\u4e2a\u5b9e\u9a8c\u7ec4\uff08Veh-Con\u3001Veh-TBI\u3001PLX-Con\u3001PLX-TBI\uff09\u7684\u7ec6\u80de\u6bd4\u4f8b\u3002\u8fd9\u91cc\uff0cPLX5622\u548cTBI\u4f9d\u8d56\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u662f\u660e\u663e\u7684\u3002\u63d0\u4f9b\u4e86\u4e00\u4e2a\u4ee3\u8868\u6027\u7684\u70ed\u56fe\uff0c\u663e\u793a\u5b9a\u4e49\u6bcf\u4e2a\u7c07\u7684\u57fa\u56e0\u8868\u8fbe\uff08\u6269\u5c55\u6570\u636e\u56fe4-1\uff09\u3002<strong>\u997c\u56fe\uff08\u56fe4C\uff09\u53cd\u6620\u4e86\u6bcf\u4e2a\u5b9e\u9a8c\u6761\u4ef6\u4e0b\u6bcf\u4e2a\u7c07\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\u6bd4\u4f8b\u3002<\/strong>\u6bcf\u4e2a\u5b9e\u9a8c\u7ec4\u4e2d\u8fc7\u5ea6\u4ee3\u8868\u7684\u7c07\u4e2d\u7684\u57fa\u56e0\u88ab\u5217\u51fa\uff0c\u5e76\u5728\u4e0e\u6240\u6709\u5176\u4ed6\u5c0f\u80f6\u8d28\u7ec6\u80de\u76f8\u6bd4\u65f6\uff0c\u663e\u8457\u9ad8\u8868\u8fbe\uff08p-adj &lt; 0.05\uff09\u3002\u4f8b\u5982\uff0c\u7c073\uff08\u7eff\u8272\uff09\u4e3b\u8981\u5728Veh-Con\u5c0f\u9f20\u7684\u76ae\u8d28\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e2d\u8868\u793a\uff08\u56fe4B\uff0cC\uff09\u3002\u7c073\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\u8868\u8fbe\u4e86\u66f4\u9ad8\u6c34\u5e73\u7684\u7a33\u6001\u5c0f\u80f6\u8d28\u7ec6\u80de\u57fa\u56e0\uff0c\u5305\u62ecCx3cr1\u548cTgfbr1\u3002\u7c072\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\uff08\u519b\u7eff\u8272\uff09\u5360\u4e86PLX-Con\u548cPLX-TBI\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u7ea650%\uff0c\u5e76\u5728Veh-TBI\u7ec4\u4e2d\u5bcc\u96c6\u3002\u7c072\u9ad8\u5ea6\u8868\u8fbe\u7f16\u7801\u6838\u7cd6\u4f53\u86cb\u767d\u7684mRNA\uff0c\u8fd9\u53ef\u80fd\u8868\u660e\u589e\u52a0\u7684\u589e\u6b96\u6216\u4ee3\u8c22\u8d1f\u62c5\u3002\u7c077\u4e5f\u88abPLX\u5904\u7406\u4e30\u5bcc\u3002\u7c077\u8868\u8fbe\u589e\u6b96\u76f8\u5173\u7684\u57fa\u56e0\uff0c\u5305\u62ec\u6cdb\u7d20\u8fde\u63a5\u9176E2C\uff08Ube2c\uff09\u548c\u7740\u4e1d\u7c92\u86cb\u767dA\uff08Cenpa\uff09\uff0c\u4ee5\u53ca\u7ec6\u80de\u9aa8\u67b6\u6210\u5206\uff0c\u5305\u62ec\u4e0e\u5fae\u7ba1\u76f8\u5173\u548c\u4e0e\u808c\u52a8\u86cb\u767d\u76f8\u5173\u7684\u57fa\u56e0\u3002\u56e0\u6b64\uff0c\u7c072\u548c7\u53ef\u80fd\u4ee3\u8868\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d3b\u8dc3\u8f6c\u6362\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\u56fe\u7247[3]-\u5403\u4e86\u5403\u4e86\" decoding=\"async\" src=\"https:\/\/cdn.ineuro.net\/cloudreve%2F2023%2F09%2F08%2FPQcqEjPH_wx2-2.png\" \/><\/figure>\n\n\n\n<p>TBI\u5bf9\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e9a\u7fa4\u7684\u5f71\u54cd\u4e0e\u589e\u52a0\u7684\u708e\u75c7\u548c1\u578b\u5e72\u6270\u7d20\u4fe1\u53f7\u6709\u5173\u3002\u4f8b\u5982\uff0c\u7c076\uff08\u84dd\u8272\uff09\u4e3b\u8981\u6765\u6e90\u4e8eVeh-TBI\u76ae\u5c42\u7684\u5c0f\u80f6\u8d28\u7ec6\u80de\uff0c\u8fd9\u4e9b\u7ec6\u80de\u9ad8\u5ea6\u8868\u8fbe\u5e72\u6270\u7d20\u5e94\u7b54\u57fa\u56e0\uff08Ifitm3\u3001Isg15\u3001Ifi27l2a\uff09\u4ee5\u53ca\u5176\u4ed6\u514d\u75ab\u76f8\u5173\u57fa\u56e0\uff08Ccl12\u3001Cd63\u3001Cd52\u3001H2-D1\u548cH2-K1\uff09\u3002\u6b64\u5916\uff0c\u7c078\uff08\u7d2b\u8272\uff09\u4e5f\u5728Veh-TBI\u7ec4\u4e2d\u5bcc\u96c6\uff0c\u5e76\u5305\u542b\u589e\u52a0\u7684\u4e0e\u5e72\u6270\u7d20\u76f8\u5173\u7684\uff08Ifi27l2a\uff09\u548c\u4e0e\u635f\u4f24\u76f8\u5173\u7684\u57fa\u56e0\uff08Cd52\u3001Flt1\u3001Tsmb4x\u3001Apoe\uff09\u3002\u53e6\u5916\uff0c\u7c079\uff08\u7c89\u7ea2\u8272\uff09\u5728Veh-TBI\u548cPLX-TBI\u7ec4\u4e2d\u5bcc\u96c6\uff0c\u5e76\u6709\u589e\u52a0\u7684Cd52\u3001Clec7a\u3001Apoe\u548cTrem2\u8868\u8fbe\u3002\u968f\u7740TBI\uff087 dpi\uff09\u7684\u589e\u52a0\uff0cVehCon\u7ec4\u4e2d\u660e\u663e\u7684\u66f4\u591a\u7a33\u6001\u57fa\u56e0\uff08\u7c073\u548c4\uff09\u5728TBI\u540e\u6210\u6bd4\u4f8b\u51cf\u5c11\u3002\u8fd9\u4e9b\u6570\u636e\u4e0e\u76ae\u8d28mRNA\u5206\u6790\u4e2d\u660e\u663e\u7684\u589e\u52a0\u7684\u5e72\u6270\u7d20\u9014\u5f84\uff08\u56fe1E\uff09\u662f\u4e00\u81f4\u7684\u3002<\/p>\n\n\n\n<p>\u56fe4D\u8fdb\u4e00\u6b65\u5f3a\u8c03\u4e86\u7c07\u7279\u5f02\u6027\u7684\u8868\u8fbe\u6a21\u5f0f\uff0c\u663e\u793a\u4e86\u4e0e\u5c0f\u80f6\u8d28\u7ec6\u80de\u7a33\u6001\u76f8\u5173\u7684\u57fa\u56e0\uff08Tgfbr1\u3001P2ry12\u3001Cx3cr1\u3001Tmem119\uff09\u3001\u5c0f\u80f6\u8d28\u7ec6\u80de\u5bf9\u635f\u4f24\u7684\u53cd\u5e94\u4e2d\u6d89\u53ca\u7684\u57fa\u56e0\uff08Trem2\u3001Apoe\u3001Clec7a\u3001Cd52\uff09\u4ee5\u53ca\u5e72\u6270\u7d20\u5e94\u7b54\u57fa\u56e0\uff08Ifitm3\u3001Irf7\u3001Ifi27l2a\u3001Stat1\uff09\u3002\u70b9\u56fe\u663e\u793a\u4e86\u6309\u7c07\u7684\u76f8\u5bf9\u57fa\u56e0\u8868\u8fbe\uff08\u989c\u8272\u5f3a\u5ea6\uff09\u548c\u6bcf\u4e2a\u7c07\u8868\u8fbe\u57fa\u56e0\u7684\u7ec6\u80de\u6bd4\u4f8b\uff08\u70b9\u5927\u5c0f\uff09\u3002\u5177\u6709\u66f4\u591a\u708e\u75c7\u57fa\u56e0\u8868\u8fbe\u7684\u7c07\u76f8\u5e94\u5730\u51cf\u5c11\u4e86\u5148\u524d\u63cf\u8ff0\u7684\u7a33\u6001\u57fa\u56e0\u8868\u8fbe\uff08Krasemann\u7b49\uff0c2017\uff09\u3002\u4f8b\u5982\uff0cTBI\u5bcc\u96c6\u7684\u7c07\uff0c\u7c076\uff0cCd52\u3001Ifitm3\u3001Irf7\u3001Ifi27l2a\u548cStat1\u7684\u8868\u8fbe\u76f8\u5bf9\u589e\u52a0\uff0c\u4e0e\u7a33\u6001\u57fa\u56e0\uff0c\u5305\u62ecTgfb1\u3001Cx3cr1\u548cTmem119\u76f8\u5e94\u5730\u51cf\u5c11\uff08\u56fe4D\uff09\u3002\u6b64\u5916\uff0c\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u7c07\uff08Krasemann\u7b49\uff0c2017; Deczkowska\u7b49\uff0c2018\uff09\uff0c\u7c079\uff0c\u6709\u589e\u5f3a\u7684Trem2\u3001Apoe\u3001Clec7a\u3001Cd52\u4e0e\u5305\u62ecTgfb1\u3001Cx3cr1\u548cTmem119\u7684\u7a33\u6001\u57fa\u56e0\u76f8\u5e94\u5730\u51cf\u5c11\uff08\u56fe4D\uff09\u3002<\/p>\n\n\n\n<p>\u5f53Veh-Con\u548cVeh-TBI\u7ec4\u5728\u6240\u6709\u7c07\u4e2d\u8fdb\u884c\u6bd4\u8f83\u65f6\uff0c66\u4e2a\u57fa\u56e0\u6709\u663e\u8457\u7684\u5dee\u5f02\u8868\u8fbe\uff08p-adj&lt;0.05\uff1b43\u4e2a\u589e\u52a0\uff0c23\u4e2a\u51cf\u5c11\uff09\u3002\u56fe4E\u663e\u793a\u4e86\u8fd9\u4e9bTBI\u4f9d\u8d56\u7684\u57fa\u56e0\u7684\u9009\u62e9\u3002\u5bf9Veh-Con\u548cVeh-TBI\u6837\u672c\u4e4b\u95f4\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e2d66\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684IPA\u4e0a\u6e38\u8c03\u8282\u56e0\u5b50\u5206\u6790\u4e0e7dpi\u65f6\u4f5c\u4e3aTBI\u529f\u80fd\u7684\u589e\u52a0\u76841\u578b\u5e72\u6270\u7d20\u4fe1\u53f7\u4e00\u81f4\u3002\u4f8b\u5982\uff0c7dpi\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e2d\u53d7\u5f71\u54cd\u7684\u4e0a\u6e38\u8c03\u8282\u56e0\u5b50\u5305\u62ec\u589e\u52a0\u7684IFNA\u3001IRF3\u3001IFNAR\u548cSTAT1\u3002\u56e0\u6b64\uff0c7dpi\u65f6\u5c0f\u80f6\u8d28\u7ec6\u80de\u4e2d\u6709\u4e00\u4e2a\u5f3a\u70c8\u7684\u4e0e\u5e72\u6270\u7d20\u76f8\u5173\u7684\u8868\u8fbe\u6a21\u5f0f\u3002\u66f4\u5177\u4f53\u5730\u8bf4\uff0c\u4e0e\u521b\u4f24\u5bcc\u96c6\u7684\u7c076\u5bf91\u578b\u5e72\u6270\u7d20\u4fe1\u53f7\u9ad8\u5ea6\u53cd\u5e94\u3002\u8fd9\u53ef\u80fd\u53cd\u6620\u4e86\u76ae\u5c427dpi\u7684\u6301\u7eed\u708e\u75c7\u548c\u7ec6\u80de\u635f\u4f24\u3002\u603b\u4f53\u800c\u8a00\uff0c\u67095\u4e2a\u5c0f\u80f6\u8d28\u7ec6\u80de\u7c07\u5728\u7279\u5b9a\u6cbb\u7597\u7ec4\u4e2d\u5bcc\u96c6\uff08\u56fe4G\uff09\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>IPA\u5206\u6790<\/p>\n\n\n\n<p>IPA \u662f\u57fa\u4e8eQIAGEN\u8fd120\u5e74\u6536\u96c6\u6574\u7406\u7684QIAGEN Knowledge Base \u5f00\u53d1\u7684\u4e00\u6b3e\u7ec4\u5b66\u6570\u636e\u5206\u5b50\u673a\u7406\u6316\u6398\u5de5\u5177\u3002\u53ef\u4ee5\u5bf9\u57fa\u56e0\u3001\u86cb\u767d\u3001\u4ee3\u8c22\u7269\u3001\u836f\u7269\u548c\u5316\u5408\u7269\u7b49\u8fdb\u884c\u751f\u7269\u5b66\u529f\u80fd\u548c\u5206\u5b50\u8c03\u63a7\u673a\u7406\u7684\u89e3\u8bfb\uff0c\u9610\u660e\u751f\u7269\u5b66\u73b0\u8c61\u80cc\u540e\u7684\u5206\u5b50\u673a\u7406\u3001\u6316\u6398\u6f5c\u5728\u7684\u751f\u7269\u5b66\u6807\u5fd7\u7269\u3002<\/p>\n\n\n\n<p>IPA\u7684\u5206\u6790\u7ed3\u679c\u53ef\u4ee5\u5206\u4e3a\u516d\u4e2a\u6a21\u5757\uff1a\u7ecf\u5178\u901a\u8def\u5206\u6790\u3001\u4e0a\u6e38\u5206\u6790\u3001\u75be\u75c5\u4e0e\u529f\u80fd\u5206\u6790\u3001\u8c03\u63a7\u6548\u5e94\u5206\u6790\u3001\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\u3001\u56fe\u5f62\u6458\u8981\u3002<\/p>\n\n\n\n<p><strong>\u4e0a\u6e38\u5206\u6790<\/strong><\/p>\n\n\n\n<p>\u5206\u6790\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u65f6\u7684\u4e00\u4e2a\u5173\u952e\u95ee\u9898\u5728\u4e8e\uff0c\u63a2\u7a76\u54ea\u4e9b\u4e0a\u6e38\u5206\u5b50\u53ef\u80fd\u9a71\u52a8\u6570\u636e\u96c6\u4e2d\u7684\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u8868\u8fbe\u53d8\u5316\u3002IPA\u4f1a\u9884\u6d4b\u54ea\u4e9b\u4e0a\u6e38\u8c03\u8282\u56e0\u5b50\u88ab\u6fc0\u6d3b\u6216\u6291\u5236\uff0c\u5bfc\u81f4\u4e86\u6570\u636e\u96c6\u4e2d\u4e0a\u8c03\u548c\u4e0b\u8c03\u7684\u57fa\u56e0\u3002<\/p>\n<\/blockquote>\n\n\n\n<h2 class=\"wp-block-heading\">5.TBI\u8bf1\u5bfc\u708e\u75c7\u548c\u53cd\u5e94\u6027\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7279\u5f81\uff0c\u800c\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u90e8\u5206\u963b\u6b62\u4e86\u8fd9\u4e00\u8fc7\u7a0b\u3002<\/h2>\n\n\n\n<p>\u5bf9Gja+\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7684\u8fdb\u4e00\u6b65\u5206\u6790\u63ed\u793a\u4e86\u6240\u6709\u56db\u4e2a\u5b9e\u9a8c\u7ec4\u4e2d\u7684\u4e03\u4e2a\uff080-6\uff09\u7ec6\u80de\u7c07\uff08\u56fe5A\uff09\u3002\u56fe5B\u548c5C\u7a81\u663e\u4e86\u5728\u7279\u5b9a\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7c07\u4e2d\u5f3a\u70c8\u8868\u8fbe\u7684\u57fa\u56e0\u3002\u4f8b\u5982\uff0c\u7c075\u4e0e\u9ad8Gfap\u8868\u8fbe\u76f8\u5173\uff0c\u4e0e\u53cd\u5e94\u6027\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e00\u81f4\uff0c\u800cSpry2\u5219\u4e0eBDNF\u4fe1\u53f7\u548c\u795e\u7ecf\u7a81\u8d77\u751f\u957f\u6709\u5173\u3002\u8fd9\u4e9b\u53d1\u73b0\u4e0e\u4ee5\u524d\u7684\u62a5\u544a\u4e00\u81f4\uff0c\u5373\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4fdd\u6301\u5f02\u8d28\u6027\u7684\u7ec6\u80de\u8868\u9762\u6807\u8bb0\uff0c\u5e76\u5728\u6210\u4ebaCNS\u4e2d\u5177\u6709\u591a\u79cd\u529f\u80fd\u3002<\/p>\n\n\n\n<p>\u57287dpi\u7684Gja+\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\uff0c\u670955\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u53d1\u751f\u4e86\u5dee\u5f02\uff08Veh-Con\u4e0eVeh-TBI\u4e4b\u95f4\uff0cp-adj&lt;0.05\uff09\u3002\u5176\u4e2d\uff0c27\u4e2a\u5728TBI\u540e\u589e\u52a0\uff08\u56fe5D\uff09\uff0c28\u4e2a\u51cf\u5c11\uff08\u56fe5E\uff09\u3002\u6240\u6709\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u90fd\u5728\u56fe5D\u548c5E\u4e2d\u8868\u793a\uff0c\u5176\u4e2d\u70b9\u56fe\u53cd\u6620\u4e86\u6309\u6cbb\u7597\u7ec4\u7684\u76f8\u5bf9\u57fa\u56e0\u8868\u8fbe\uff08\u989c\u8272\u5f3a\u5ea6\uff09\u4ee5\u53ca\u6bcf\u4e2a\u7c07\u4e2d\u8868\u8fbe\u57fa\u56e0\u7684\u7ec6\u80de\u6bd4\u4f8b\uff08\u70b9\u7684\u5927\u5c0f\uff09\u3002\u589e\u52a0\u7684\u57fa\u56e0\u5305\u62ec\u4e0e\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u53cd\u5e94\u6027\u5728\u4f24\u5bb3\/\u75be\u75c5\u4e2d\u76f8\u5173\u7684Gfap\u3001Apoe\u548cClu\u3002\u6b64\u5916\uff0c\u4e0e\u751f\u957f\u56e0\u5b50\u4fe1\u53f7\uff08Vegfa, Fgfr3\uff09\u548c\u7ec6\u80de\u5916\u57fa\u8d28\u91cd\u5851\uff08Bcan\uff09\u4ee5\u53ca\u661f\u5f62\u80f6\u8d28\u7ec6\u80deNotch\u4fe1\u53f7\uff08Lfng, Nrarp\uff09\u76f8\u5173\u7684\u57fa\u56e0\u7684\u8868\u8fbe\u51cf\u5c11\u3002\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u88ab\u7528\u4e8eIPA\u5206\u6790\uff0c\u4e09\u4e2a\u4e0a\u6e38\u8c03\u8282\u5668\u5728\u4f24\u5bb3\u540e\u663e\u8457\u4e0d\u540c\uff08p&lt;0.05\uff1b\u56fe5F\uff09\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u4e0eTBI\u8bf1\u5bfc\u7684\u57fa\u56e0\u8868\u8fbe\u4e00\u81f4\uff0cCEBPB\u4fe1\u53f7\u589e\u52a0\uff0c\u8fd9\u662f\u4e00\u4e2a\u8c03\u8282\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\u708e\u75c7\u57fa\u56e0\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\u3002\u57287dpi\u768455\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u4e2d\uff0c82%\uff0845\u4e2a\u57fa\u56e0\uff09\u7684\u8fd9\u79cd\u7279\u5f81\u5728PLX-TBI\u7ec4\u4e0eVeh-TBI\u76f8\u6bd4\u5f97\u5230\u4e86\u9006\u8f6c\uff08p&lt;0.05\uff1b\u56fe5G\uff09\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u4e9b\u6570\u636e\u8868\u660e\uff0c\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u91c7\u7eb3\u4e86\u4e00\u4e2a\u708e\u75c7\u548c\u53cd\u5e94\u6027\u7684\u7279\u5f81\uff0c\u5176\u7279\u70b9\u662f\u589e\u52a0\u7684\u708e\u75c7\u548c\u51cf\u5c11\u7684\u7a33\u6001\u57fa\u56e0\u8868\u8fbe\uff0c\u800c\u8fd9\u79cd\u7279\u5f81\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u5f97\u5230\u4e86\u51cf\u5f31\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\u56fe\u7247[4]-\u5403\u4e86\u5403\u4e86\" decoding=\"async\" src=\"https:\/\/cdn.ineuro.net\/cloudreve%2F2023%2F09%2F08%2FBNNUiFf4_wx2-3.png\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">6.\u9ad3\u9798\u5f62\u6210\u7684\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u5728TBI\u540e\u53d1\u751f\u4e0e\u9ad3\u9798\u5f62\u6210\u548cCNS\u635f\u4f24\u53cd\u5e94\u4e00\u81f4\u7684\u8f6c\u5f55\u53d8\u5316\uff0c\u800c\u8fd9\u4e00\u53d8\u5316\u88ab\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u8017\u6240\u51cf\u5f31\u3002<\/h2>\n\n\n\n<p>\u8fdb\u4e00\u6b65\u5206\u6790Plp1+\u7ec6\u80de\u63ed\u793a\u4e86\u4e5d\u4e2a\uff080-8\uff09\u7c07\uff08\u56fe6A\uff09\u3002\u56fe6B\u548c6C\u7a81\u51fa\u663e\u793a\u4e86\u4e0e\u5176\u4ed6Plp11\u7ec6\u80de\u76f8\u6bd4\u5728\u7279\u5b9a\u79cd\u7fa4\u4e2d\u589e\u52a0\u7684\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u57fa\u56e0\u3002\u4f8b\u5982\uff0c<strong>Mobp\u548cMog\u5728\u9ad3\u9798\u5f62\u6210\u7684\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u7c07\uff080-4\uff0c6\uff0c8\uff09\u4e2d\u9ad8\u5ea6\u8868\u8fbe\uff0c\u800cPdgfra\u5728\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u524d\u4f53\u7ec6\u80de\uff08OPCs; \u7c075\uff09\u4e2d\u9ad8\u5ea6\u8868\u8fbe\u3002\u7c077\u7531\u8868\u8fbe\u4e0e\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u6210\u719f\u6709\u5173\u7684\u57fa\u56e0\uff08\u5982Slc1a1, Gpr17, Bcan\uff09\u7684\u7ec6\u80de\u7ec4\u6210\uff0c\u5e76\u964d\u4f4e\u4e86\u9ad3\u9798\u5f62\u6210\u57fa\u56e0Mog\u548cMobp\u7684\u8868\u8fbe<\/strong>\u3002<\/p>\n\n\n\n<p>TBI\u540e\uff0c\u5728\u9ad3\u9798\u5f62\u6210\u7684\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u4e2d\u670939\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff08Veh-Con\u4e0eVeh-TBI\u6bd4\u8f83\uff0cpadj&lt;0.05\uff09\u3002\u5176\u4e2d\uff0c15\u4e2a\u57287dpi\u65f6\u589e\u52a0\uff08\u56fe6D\uff09\uff0c24\u4e2a\u51cf\u5c11\uff08\u56fe6E\uff09\u3002\u6240\u6709\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u90fd\u5728\u56fe6D\u548c6E\u4e2d\u8868\u793a\uff0c\u5176\u4e2d\u70b9\u56fe\u53cd\u6620\u4e86\u6309\u6cbb\u7597\u7ec4\u7684\u76f8\u5bf9\u57fa\u56e0\u8868\u8fbe\uff08\u989c\u8272\u5f3a\u5ea6\uff09\u4ee5\u53ca\u6bcf\u4e2a\u7c07\u4e2d\u8868\u8fbe\u57fa\u56e0\u7684\u7ec6\u80de\u6bd4\u4f8b\uff08\u70b9\u7684\u5927\u5c0f\uff09\u3002\u589e\u52a0\u7684\u57fa\u56e0\u5305\u62ec\u90a3\u4e9b\u7f16\u7801\u56db\u8de8\u819c\u86cb\u767d\uff08\u5982Cd9, Cd81\uff09\u7684\u57fa\u56e0\uff0c\u8fd9\u4e9b\u57fa\u56e0\u4e0e\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u7684\u53d1\u5c55\u6709\u5173\uff0c\u5e76\u4e0e\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u5bf9CNS\u635f\u4f24\/\u5e94\u6fc0\u7684\u53cd\u5e94\u76f8\u5173\u7684\u57fa\u56e0\uff08\u5982Apod, Hspa8\uff09\u3002\u572839\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u4e2d\uff0c\u670932\u4e2a\u88ab\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u6240\u9006\u8f6c\uff08\u56fe6F\uff09\u3002\u5c3d\u7ba1\u5728\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u4e2d\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u6570\u91cf\u76f8\u5bf9\u8f83\u5c11\uff0c\u4f46\u5728TBI\u540e\u8fd8\u662f\u53ef\u4ee5\u68c0\u6d4b\u5230\u53d6\u51b3\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u53d8\u5316\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\u56fe\u7247[5]-\u5403\u4e86\u5403\u4e86\" decoding=\"async\" src=\"https:\/\/cdn.ineuro.net\/cloudreve%2F2023%2F09%2F08%2FGDLMHcld_wx2-4.png\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">7.TBI\u4e0e7 dpi\u7684\u795e\u7ecf\u5143\u7a33\u6001\u964d\u4f4e\u6709\u5173\uff0c\u800c\u8fd9\u79cd\u964d\u4f4e\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u5f97\u5230\u4e86\u7f13\u89e3\u3002<\/h2>\n\n\n\n<p>\u63a5\u4e0b\u6765\uff0c\u5206\u6790\u4e86Meg3+\u76ae\u5c42\u795e\u7ecf\u5143(2878\u4e2a)\uff0c\u5e76\u786e\u5b9a\u4e86\u5341\u4e00\u4e2a(0-10)\u4e0d\u540c\u7684\u7c07\uff08\u56fe7A\uff1b\u6269\u5c55\u6570\u636e\u56fe7-1\u663e\u793a\u7684\u8868\u8fbe\u70ed\u56fe\uff09\u3002\u56fe7B\u7a81\u51fa\u663e\u793a\u4e86\u795e\u7ecf\u5143\u7279\u5f02\u6027\u57fa\u56e0\uff08Meg3\uff0cCamk2n1\uff09\u548c\u4e0e\u7a81\u89e6\u795e\u7ecf\u5143\uff08Thy1\uff0cApp\uff09\u3001\u6291\u5236\u6027\u795e\u7ecf\u5143\uff08Gad1\uff09\u6216\u8f74\u7a81\u8fd0\u8f93\uff08Kif5a\uff09\u76f8\u5173\u7684\u57fa\u56e0\u3002\u56db\u4e2a\u5b9e\u9a8c\u7ec4\u4e2d\u6bcf\u4e2a\u7c07\u7684\u6bd4\u4f8b\u5728\u7c07\u4e4b\u95f4\u76f8\u5bf9\u4e00\u81f4\uff08\u6269\u5c55\u6570\u636e\u56fe7-2\uff09\u3002<\/p>\n\n\n\n<p>\u603b\u4f53\u4e0a\uff0c7 dpi\u7684Meg3+\u76ae\u5c42\u795e\u7ecf\u5143\u4e2d\u6709233\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff08Veh-Con\u4e0eVeh-TBI\u6bd4\u8f83\uff09\u3002\u5176\u4e2d\uff0c95%\uff08221\u4e2a\u57fa\u56e0\uff09\u51cf\u5c11\uff0c5%\uff0812\u4e2a\u57fa\u56e0\uff09\u589e\u52a0\uff08p-adj&lt;0.05\uff1b\u56fe7C\uff09\u3002<\/p>\n\n\n\n<p>\u5229\u7528\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u8fdb\u884cIPA\u5206\u6790\uff0c\u51e0\u4e2a\u7ecf\u5178\u901a\u8def\u7684\u6291\u5236\u662f\u660e\u663e\u7684\uff08\u56fe7D\uff09\u3002\u4f8b\u5982\uff0c7 dpi\u65f6\u9499\u4fe1\u53f7\u3001\u7a81\u89e6\u751f\u6210\u548c\u7a81\u89e6\u957f\u65f6\u7a0b\u589e\u5f3a\u90fd\u88ab\u6291\u5236\uff08\u6bcf\u4e2a\u7684p&lt;0.00003\uff09\u3002\u6b64\u5916\uff0c7 dpi\u65f6\u7a81\u89e6\u957f\u65f6\u7a0b\u6291\u5236\u548c\u8c37\u6c28\u9178\u53d7\u4f53\u4fe1\u53f7\u589e\u52a0\uff08p&lt;0.0001\uff09\u3002\u76ae\u5c42\u795e\u7ecf\u5143\u7684\u4e0a\u6e38\u8c03\u63a7\u56e0\u5b50\u5206\u6790\u663e\u793a\u5728\u56fe7E\u4e2d\uff08p&lt;0.05\uff1b\u7edd\u5bf9z\u5206\u6570&gt;2\uff09\u3002<\/p>\n\n\n\n<p>TBI\u540e\u7684\u795e\u7ecf\u5143\u57fa\u56e0\u8868\u8fbe\u4e0e\u751f\u957f\u548c\u5b58\u6d3b\uff08TGFB\uff0cMKNK1\uff0cIGF1\uff09\u3001\u795e\u7ecf\u7a81\u8d77\u751f\u957f\uff08NRF1\uff09\u548c\u7ec6\u80de\u5e94\u6fc0\u8c03\u63a7\uff08XBP1\uff0cATF6\uff0cNFE2L2\uff09\u76f8\u5173\u7684\u8c03\u63a7\u56e0\u5b50\u7684\u51cf\u5c11\u8868\u8fbe\u4e00\u81f4\u3002\u9a71\u52a8\u8fd9\u4e9b\u901a\u8def\u7ea7\u53d8\u5316\u7684\u5173\u952e\u57fa\u56e0\u5728\u56fe7F\u4e2d\u663e\u793a\uff0c\u901a\u8fc7\u6cbb\u7597\u7ec4\uff08\u989c\u8272\u5f3a\u5ea6\uff09\u548c\u6bcf\u4e2a\u7c07\u4e2d\u8868\u8fbe\u57fa\u56e0\u7684\u7ec6\u80de\u6bd4\u4f8b\uff08\u70b9\u7684\u5927\u5c0f\uff09\u8868\u793a\u76f8\u5bf9\u57fa\u56e0\u8868\u8fbe\u3002\u4e0eVeh-Con\u76f8\u6bd4\uff0cVeh-TBI\u4e2d\u6240\u6709\u7f16\u7801\u8c37\u6c28\u9178AMPA\u53d7\u4f53\u4e9a\u57fa\u7684\u57fa\u56e0\uff08Gria2\uff0cGria3\uff0cGria4\uff09\u90fd\u589e\u52a0\uff08p-adj&lt;0.05\uff1b\u56fe7E\uff09\u3002\u6240\u6709\u5176\u4ed6\u795e\u7ecf\u5143\u57fa\u56e0\u90fd\u88ab\u6291\u5236\u3002\u4f8b\u5982\uff0c\u4e0e\u9499\u7a33\u6001\u548c\u4fe1\u53f7\u4f20\u5bfc\u76f8\u5173\u7684\u57fa\u56e0\uff08Ryr2\uff0cCalm1\uff0cCaly\uff09\u5728TBI\u540e\u7684\u76ae\u5c42\u795e\u7ecf\u5143\u4e2d\u51cf\u5c11\u3002<\/p>\n\n\n\n<p>\u4e0eVeh-TBI\u76f8\u6bd4\uff0c\u5927\u591a\u6570\u57fa\u56e0\u8868\u8fbe\u7684\u53d8\u5316\u5728PLX-TBI\u7ec4\u4e2d\u5f97\u5230\u4e86\u9006\u8f6c\uff08p&lt;0.05\uff09\u3002\u4f8b\u5982\uff0c\u7528PLX5622\u6d88\u8017\u5c0f\u80f6\u8d28\u7ec6\u80de\u51cf\u5f31\u4e86\u76ae\u5c42\u795e\u7ecf\u5143\u4e2d95%\u7684TBI\u4f9d\u8d56\u6027\u8f6c\u5f55\u53d8\u5316\uff08\u56fe7G\uff09\u3002\u5728TBI\u540e\u5dee\u5f02\u8868\u8fbe\u7684233\u4e2a\u57fa\u56e0\u4e2d\uff0c193\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\u6062\u590d\u5230Veh-Con\u6c34\u5e73\uff0c28\u4e2a\u90e8\u5206\u9006\u8f6c\u3002\u6bcf\u4e2a\u7c7b\u522b\u7684\u9009\u5b9a\u57fa\u56e0\u5728\u56fe7G\u4e2d\u663e\u793a\u3002\u4e0e\u795e\u7ecf\u5143\u53ef\u5851\u6027\u548c\u56ca\u6ce1\u8fd0\u8f93\u76f8\u5173\u7684\u57fa\u56e0\uff08Nsg1\uff0cSyt5\uff0cArf5\uff09\u4ee5\u53ca\u795e\u7ecf\u5143\u8c03\u63a7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u57fa\u56e0\uff08Cx3cl1\uff0cCd200\uff09\u5728TBI\u540e\u88ab\u6291\u5236\uff08p-adj&lt;0.05\uff09\uff0c\u5e76\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u5f97\u5230\u9006\u8f6c\uff08p&lt;0.05\uff09\u3002\u603b\u4f53\u6765\u8bf4\uff0c\u8fd9\u4e9b\u6570\u636e\u8868\u660e\uff0c\u5728TBI\u540e7\u5929\uff0c\u652f\u6301\u795e\u7ecf\u5143\u7a33\u6001\u529f\u80fd\u7684\u57fa\u56e0\u5728\u5c0f\u80f6\u8d28\u7ec6\u80de\u4f9d\u8d56\u7684\u65b9\u5f0f\u4e0b\u88ab\u6291\u5236\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\u56fe\u7247[6]-\u5403\u4e86\u5403\u4e86\" decoding=\"async\" src=\"https:\/\/cdn.ineuro.net\/cloudreve%2F2023%2F09%2F09%2FO51omh7I_wx2-5.png\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">8.TBI\u540e\u7684\u6811\u7a81\u590d\u6742\u6027\u51cf\u5c11\u53ef\u4ee5\u901a\u8fc7\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u9664\u5f97\u5230\u7f13\u89e3<\/h2>\n\n\n\n<p>\u7814\u7a76\u4eba\u5458\u76ee\u524d\u548c\u4e4b\u524d\u7684RNA\u548cscRNAseq\u5206\u6790\u7814\u7a76\u8868\u660e\uff0c\u5c0f\u80f6\u8d28\u7ec6\u80de\u4ecb\u5bfc\u6162\u6027\u708e\u75c7\u548c\u6f5c\u5728\u7684\u75c5\u7406\uff08Witcher\u7b49\uff0c2018\uff09\uff0c\u5e76\u4e14\u4e0e\u795e\u7ecf\u5143\u7a33\u6001\u76f8\u5173\u7684\u9014\u5f84\u51cf\u5c11\u4e86\u3002\u5176\u4ed6\u7814\u7a76\u8868\u660e\u5c0f\u80f6\u8d28\u7ec6\u80de\u53c2\u4e0e\u6811\u7a81\u91cd\u5851\u548c\u8c03\u8282\u7a81\u89e6\uff08Schafer\u7b49\uff0c2012\uff09\u3002\u56e0\u6b64\uff0c\u5728TBI\uff087 dpi\uff09\u540e\uff0c\u7814\u7a76\u4eba\u5458\u8bc4\u4f30\u4e86\u6709\u6216\u6ca1\u6709\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u7684Thy1-YFP-H\u5c0f\u9f20\u76ae\u5c42\u7684\u795e\u7ecf\u5143\u5f62\u6001\u548c\u810a\u7a81\u5bc6\u5ea6\uff08\u56fe8A\uff09\u3002\u8fd9\u91cc\uff0c\u7814\u7a76\u4eba\u5458\u5c55\u793a\u4e86\u5728\u76ae\u5c427 dpi\u7684\u795e\u7ecf\u5143\u590d\u6742\u6027\uff08Sholl\u4ea4\u53c9\u70b9\uff09\u51cf\u5c11\u4e86(F(3,80) = 2.912, p = 0.0273\uff1b\u56fe8B-D)\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u5728TBI\u4e4b\u524d\u6d88\u9664\u4e86\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u5c0f\u9f20\u4e2d\uff0c\u8fd9\u79cd\u6548\u5e94\u4e0d\u5b58\u5728\u3002\u7814\u7a76\u4eba\u5458\u8fd8\u8bc4\u4f30\u4e86Thy1-YFP-H\u5c0f\u9f20\u7684\u6811\u7a81\u810a\u7a81\u5bc6\u5ea6\uff08\u56fe8E\uff09\uff0c\u5e76\u4e14\u5728TBI\u540e\u603b\u6811\u7a81\u810a\u7a81\u5bc6\u5ea6\u6ca1\u6709\u5dee\u5f02\uff08\u56fe8F\uff09\u3002\u7136\u800c\uff0c7 dpi\u7684\u8611\u83c7\uff08\u6216\u6210\u719f\u7684\uff09\u810a\u7a81\u786e\u5b9e\u663e\u8457\u51cf\u5c11\uff08F(3,57) = 0.4471, p = 0.0169\uff1b\u56fe8G\uff09\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u77ed\u7c97\u7684\uff08\u4e0d\u6210\u719f\u7684\uff09\u810a\u7a81\u76f8\u5e94\u589e\u52a0\uff08F(3,57) = 0.4471, p = 0.0245\uff1b\u56fe8G\uff09\u3002\u5728PLX-TBI\u5c0f\u9f20\u4e2d\uff0c\u672a\u68c0\u6d4b\u5230TBI\uff08Veh-TBI\uff09\u5f15\u8d77\u7684\u8611\u83c7\u578b\uff08\u6210\u719f\u578b\uff09\u68d8\u7684\u51cf\u5c11\u548c\u77ed\u7c97\u578b\uff08\u672a\u6210\u719f\u578b\uff09\u68d8\u7684\u589e\u52a0\uff0c\u8fd9\u8868\u660e\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u6d88\u9664\u963b\u6b62\u4e86TBI\u76f8\u5173\u7684\u76ae\u5c42\u6811\u7a81\u57287 dpi\u65f6\u7684\u91cd\u5851\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">9.\u4e0eTBI\u76f8\u5173\u768430 dpi CAP\u635f\u4f24\u53d6\u51b3\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de<\/h2>\n\n\n\n<p>\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e867 dpi\u548c30 dpi\u708e\u75c7\u548c\u795e\u7ecf\u75c5\u7406\u7684\u65b0\u578bmRNA\u8bc1\u636e\uff0c\u5e76\u8bc1\u660eTBI\u540e\u5c0f\u80f6\u8d28\u7ec6\u80de\u4f1a\u5f71\u54cd\u795e\u7ecf\u5143RNA\u7684\u8868\u8fbe\u3002\u80fc\u80dd\u4f53\u5305\u542b\u6765\u81ea\u76ae\u5c42\u5c42\u7684\u795e\u7ecf\u5143\u7684\u8f74\u7a81\uff0c\u8fd9\u4e9b\u795e\u7ecf\u5143\u5728\u5f25\u6f2b\u6027TBI\u540e\u53d7\u5230\u635f\u4f24\uff08Ramos\u7b49\uff0c2008\uff09\u3002\u56e0\u6b64\uff0c\u7814\u7a76\u4eba\u5458\u5728\u6709\u6216\u65e0\u5c0f\u80f6\u8d28\u7ec6\u80de\u7684\u60c5\u51b5\u4e0b\u68c0\u67e5\u4e867\u548c30 dpi\u901a\u8fc7\u80fc\u80dd\u4f53\u7684\u795e\u7ecf\u8fde\u63a5\u6027\uff08\u56fe9A\uff09\u3002CAPs\u5728\u80fc\u80dd\u4f53\u7684\u5927\u8111\u5207\u7247\u4e2d\u88ab\u523a\u6fc0\u548c\u8bb0\u5f55\uff08\u56fe9A\uff09\u3002\u4ee3\u8868\u6027\u7684\u8ff9\u7ebf\u663e\u793a\u4e86\u5177\u6709\u63a7\u5236\u548cTBI\u7684\u52a8\u4f5c\u7535\u4f4d\u7684\u4e0d\u540c\u7684N1\u548cN2\u7ec4\u6210\u90e8\u5206\u3002\u8fd9\u4e9b\u6570\u636e\u88ab\u89e3\u91ca\u4e3a\u8868\u660eN1\u4ee3\u8868\u5feb\u901f\u4f20\u5bfc\u7ea4\u7ef4\uff08\u5927\u7684\uff0c\u9ad3\u9798\u5316\uff09\u548cN2\u4ee3\u8868\u6162\u901f\u4f20\u5bfc\u7ea4\u7ef4\uff08\u5c0f\u7684\uff0c\u65e0\u9ad3\u9798\u5316\uff09\u3002\u6bcf\u4e2a\u6cbb\u7597\u7ec4\u7684\u52a8\u4f5c\u7535\u4f4d\u7684N1\u548cN2\u7ec4\u6210\u90e8\u5206\u57287\u548c30 dpi\u4e0a\u663e\u793a\uff08\u56fe9B,C\uff09\u30027\u548c30 dpi\u7684N2 CAPs\u503e\u5411\u4e8e\u7531\u4e8eTBI\u800c\u51cf\u5c11\uff08\u5404p=0.08\uff1b\u56fe9C\uff09\u3002TBI\u7684\u4e3b\u8981\u6548\u5e94\u662f\u57287 dpi\u548c30 dpi\u6291\u5236N2\/N1\u6bd4\u7387\uff08F(4,32)=5.5\uff0cp= 0.002\uff1b\u56fe9D\uff09\u3002\u4e8b\u540e\u5206\u6790\u8bc1\u5b9e\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u63a5\u53d7TBI\u7684\u5c0f\u9f20\u57287 dpi\u548c30 dpi\u7684N2\/ N1\u6bd4\u7387\u51cf\u5c11\uff087dpi\u7684p&lt;0.001\uff1b30dpi\u7684p= 0.018\uff09\u3002\u57287dpi\u65f6\uff0cN2\/N1\u6bd4\u7387\u7684\u6291\u5236\u4e0d\u53d7\u5c0f\u80f6\u8d28\u7ec6\u80de\u6d88\u8017\u7684\u5f71\u54cd\uff08PLX-TBI 7dpi\u4e0e\u5bf9\u7167p&lt;0.001\uff09\u3002\u523030 dpi\u65f6\uff0c tbi\u8bf1\u5bfc\u7684N2\/N1 \u6bd4\u503c\u5728plx\u6cbb\u7597\u5c0f\u9f20\u4e2d\u4e0d\u518d\u660e\u663e\u964d\u4f4e(PLX-TBI 30 dpi vs\u5bf9\u7167\u7ec4p = 0.712)\u3002\u6700\u5927\u523a\u6fc0\u4e0b\uff082 mA\uff09\uff0cTBI\u7684\u6548\u5e94\u53ca\u572830 dpi\u7684PLX\u9006\u8f6c\u4f5c\u7528\u663e\u800c\u6613\u89c1\uff0cPLX-TBI\u5c0f\u9f20\u7684N2\/N1\u6bd4\u7387\u5904\u4e8e\u5bf9\u7167\u6c34\u5e73\uff0c\u4f46Veh-TBI\u5c0f\u9f20\u5219\u4e0d\u662f\uff08p= 0.02\uff1b\u56fe9E\uff09\u3002\u56e0\u6b64\uff0cTBI\u540eCAP\u5728\u6162\u6027\u65f6\u95f4\u70b9\uff0830 dpi\uff09\u7684\u635f\u4f24\u53d6\u51b3\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u3002<\/p>\n\n\n\n<p>\u4e0eTBI\u76f8\u5173\u768430 dpi\u8bb0\u5fc6\u7f3a\u9677\u53d6\u51b3\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u3002\u7814\u7a76\u4eba\u5458\u5bfb\u6c42\u786e\u5b9a\u7531\u5c0f\u80f6\u8d28\u7ec6\u80de\u5f15\u8d77\u7684\u76ae\u5c42\u4f9d\u8d56\u7684\u611f\u89c9\u8fd0\u52a8\u548c\u8ba4\u77e5\u884c\u4e3a\u572830 dpi\u5185\u662f\u5426\u53d7\u5230\u5f71\u54cd\uff08\u56fe10A\uff09\u3002\u4e3a\u786e\u5b9a\u611f\u89c9\u8fd0\u52a8\u529f\u80fd\uff0c\u4f7f\u7528\u4e86\u52a0\u901f\u7684Rotarod\u548c\u6c34\u5e73\u6746\u6d4b\u8bd5\u3002\u8fd9\u4e9b\u6d4b\u8bd5\u9700\u8981\u6574\u5408\u611f\u89c9\u548c\u8fd0\u52a8\u8f93\u5165\uff0c\u5e76\u4f9d\u8d56\u4e8e\u5b8c\u6574\u7684\u76ae\u5c42\u529f\u80fd\uff08Cao\u7b49\uff0c2015\uff09\u3002\u7136\u800c\uff0c\u52a0\u901f\u7684Rotarod\uff08\u56fe10B\uff09\u6216mFPI\u540e\u9003\u79bb\u6c34\u5e73\u6746\u6d4b\u8bd5\u7684\u6f5c\u4f0f\u671f\uff08\u56fe10C\uff09\u4e2d\uff0cTBI\u6216PLX5622\u90fd\u6ca1\u6709\u5f71\u54cd\u611f\u89c9\u8fd0\u52a8\u529f\u80fd\u3002\u8fd8\u68c0\u67e5\u4e86\u5176\u4ed6\u7684\u611f\u89c9\u8fd0\u52a8\u884c\u4e3a\uff0c\u4f46\u6ca1\u6709\u68c0\u6d4b\u5230TBI\u6216PLX\u7684\u6301\u4e45\u6548\u5e94\uff08\u6570\u636e\u672a\u663e\u793a\uff09\u3002\u56e0\u6b64\uff0c\u5f25\u6f2b\u6027TBI\u540e\uff0c\u6ca1\u6709\u4e3b\u8981\u7684\u957f\u671f\u8fd0\u52a8\u6216\u5e73\u8861\u7f3a\u9677\u3002\u572830\u548c31 dpi\uff0c\u5206\u522b\u786e\u5b9a\u4e86NOL\u548c\u65b0\u7269\u4f53\u8bc6\u522b\uff08NOR\uff09\u3002NOL\/NOR\u662f\u4f9d\u8d56\u4e8e\u5b8c\u6574\u7684\u6d77\u9a6c\u548c\u76ae\u5c42\u529f\u80fd\u7684\u8ba4\u77e5\u8bc4\u4f30\uff08Antunes\u548cBiala\uff0c2012\uff09\u3002\u6d4b\u8bd5\u7684\u7b2c\u4e00\u9636\u6bb5\u68c0\u67e5\u4e86\u7269\u4f53\u7684\u4f4d\u7f6e\uff08\u6d77\u9a6c\uff09\uff0c\u7b2c\u4e8c\u9636\u6bb5\u68c0\u67e5\u4e86NOR\uff08\u76ae\u5c42\uff1b\u56fe10A\uff09\u3002\u5728\u4efb\u4f55\u6d4b\u8bd5\u9636\u6bb5\uff0c\u56db\u4e2a\u5b9e\u9a8c\u7ec4\u4e4b\u95f4\u5728\u63a2\u7d22\u7684\u603b\u65f6\u95f4\u4e0a\u6ca1\u6709\u663e\u8457\u5dee\u5f02\uff08\u56fe10D,G\uff09\u3002\u5728\u201c\u4f4d\u7f6e\u201d\u6d4b\u8bd5\u9636\u6bb5\uff0cTBI\u548cPLX\u5e72\u9884\u4e4b\u95f4\u6709\u4e00\u79cd\u76f8\u4e92\u4f5c\u7528\u7684\u503e\u5411\uff08F(1,40)=3.65\uff0cp= 0.063\uff09\u3002\u4e8b\u540e\u5206\u6790\u8bc1\u5b9e\uff0c\u4e0e\u6240\u6709\u5176\u4ed6\u7ec4\u76f8\u6bd4\uff0cVeh-TBI\u5c0f\u9f20\u5728\u65b0\u7684\u4f4d\u7f6e\u4e0a\u82b1\u8d39\u7684\u65f6\u95f4\u76f8\u5bf9\u8f83\u5c11\uff0c\u5305\u62ec\u4e0ePLX-TBI\u5c0f\u9f20\u76f8\u6bd4\uff08\u4e0eVeh-Con p&lt;0.001\uff1b\u4e0ePLX-Con p = 0.001\uff1b\u4e0ePLX-TBI p = 0.026\uff1b\u56fe10E\uff09\u3002\u8fd9\u4e9b\u6548\u5e94\u5728NOL\u7684\u9274\u522b\u6307\u6570\u4e2d\u4e5f\u5f88\u660e\u663e\uff08\u56fe10F\uff09\uff0c\u8868\u660eVeh-TBI\u5c0f\u9f20\u5728\u719f\u6089\u7684\u4f4d\u7f6e\u4e0a\u4e0e\u7269\u4f53\u5171\u5ea6\u7684\u65f6\u95f4\u660e\u663e\u66f4\u957f\u3002\u4e0e\u65b0\u4f4d\u7f6e\u8bd5\u9a8c\u76f8\u4f3c\uff0cTBI\u548cPLX\u7ed9\u836f\u4e4b\u95f4\u5b58\u5728\u76f8\u4e92\u4f5c\u7528\uff08F(1,39) = 18.7\uff0cp = 0.0001\uff09\u7528\u4e8eNOR\u6d4b\u8bd5\u7684\u65b9\u9762\u3002\u4e8b\u540e\u5206\u6790\u8bc1\u5b9e\uff0c\u4e0e\u6240\u6709\u5176\u4ed6\u7ec4\u76f8\u6bd4\uff0cVeh-TBI\u5c0f\u9f20\u63a2\u67e5\u65b0\u7269\u4f53\u7684\u65f6\u95f4\u8f83\u5c11\uff0c\u5305\u62ec\u4e0ePLX-TBI\u7ec4\u76f8\u6bd4\uff08\u4e0eVeh-Con p&lt;0.001\uff1b\u4e0ePLX-Con p&lt;0.001\uff1b\u4e0ePLX-TBI p&lt;0.001\uff1b\u56fe10H\uff09\u3002\u8fd9\u4e9b\u5dee\u5f02\u5728Veh-TBI\u5c0f\u9f20\u4e0e\u6240\u6709\u5176\u4ed6\u7ec4\u76f8\u6bd4\u90fd\u88ab\u6291\u5236\u4e86\u7684\u65b0\u7269\u4f53\u7684\u9274\u522b\u6307\u6570\u4e2d\u53cd\u6620\u51fa\u6765\uff08\u56fe10I\uff09\u3002PLX-TBI\u5c0f\u9f20\u6ca1\u6709\u8fd9\u4e9b\u5728\u63a2\u67e5\u65b0\u7269\u4f53\u7684\u65f6\u95f4\u6216\u9274\u522b\u6307\u6570\u4e2d\u7684\u51cf\u5c11\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u4e9b\u6570\u636e\u8868\u660e\uff0cTBI\u5f15\u8d77\u7684\u76ae\u5c42\/\u6d77\u9a6c\u8bb0\u5fc6\u572830 dpi\u65f6\u4e0b\u964d\uff0c\u8fd9\u53d6\u51b3\u4e8e\u5c0f\u80f6\u8d28\u7ec6\u80de\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6587\u732e\u5b66\u4e60 Title\uff1aTraumatic Brain Injury Causes Chronic Cortic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,23],"tags":[28,25,26,29],"topics":[],"_links":{"self":[{"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/posts\/515"}],"collection":[{"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/comments?post=515"}],"version-history":[{"count":1,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/posts\/515\/revisions"}],"predecessor-version":[{"id":516,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/posts\/515\/revisions\/516"}],"wp:attachment":[{"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/media?parent=515"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/categories?post=515"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/tags?post=515"},{"taxonomy":"topics","embeddable":true,"href":"https:\/\/blog.ineuro.net\/index.php\/wp-json\/wp\/v2\/topics?post=515"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}